The measurement of nitric oxide production by cultured endothelial cells

被引：15

作者：

Hart, CM ^{[1
]}

Kleinhenz, DJ

Dikalov, SI

Boulden, BM

Dudley, SC

机构：

[1] Emory Univ, Atlanta VA Med Ctr, Dept Med, Atlanta, GA 30033 USA

[2] Emory Univ, Dept Med, Atlanta, GA 30322 USA

[3] Georgia Inst Technol, Wallace H Coulter Dept Biomed Engn, Atlanta, GA 30033 USA

来源：

NITRIC OXIDE, PT E | 2005年 / 396卷

关键词：

D O I：

10.1016/S0076-6879(05)96042-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nitric oxide (NO) produced by vascular endothelial cells (ECs) plays a critical role in normal vascular physiology. Important insights into mechanisms regulating the production of endothelial NO have been derived from in vitro studies employing cultured ECs. Although many techniques for the detection of NO have been described, many of these methodslack adequate sensitivity to detect the small amount of NO produced by cultured ECs. In this chapter, we describe three protocols that employ chemiluminescence, electron spin resonance, or electrochemical techniques to permit the reliable detection of EC NO production.

引用

页码：502 / 514

页数：13

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