Generation of new enzymes via covalent modification of existing proteins

被引：214

作者：

Qi, DF ^{[1
]}

Tann, CM ^{[1
]}

Haring, D ^{[1
]}

Distefano, MD ^{[1
]}

机构：

[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA

来源：

CHEMICAL REVIEWS | 2001年 / 101卷 / 10期

关键词：

D O I：

10.1021/cr000059o

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The different approaches for chemically modifying enzymes and proteins to obtain catalysts with either modified or completely new catalytic activities were described. With the advent of site directed mutagenesis, it is possible to introduce cysteine residues that can be introduced at any desired location and subsequently functionalized with a wide range reagents. Significant rate of catalytic acceleration was obtained in some cases.

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页码：3081 / 3111

页数：31

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