Application of magnetic chromatography to the isolation of lysosomes from fibroblasts of patients with lysosomal storage disorders

被引：76

作者：

Diettrich, O

Mills, K

Johnson, AW

Hasilik, A

Winchester, BG

机构：

[1] Univ London, Inst Child Hlth, London WC1N 1EH, England

[2] Inst Physiol Chem, D-35043 Marburg, Germany

来源：

FEBS LETTERS | 1998年 / 441卷 / 03期

基金：

英国惠康基金;

关键词：

lysosome; purification; iron dextran; infantile sialic acid storage disorder; lysosomal storage disease; two-dimensional polyacrylamide gel electrophoresis;

D O I：

10.1016/S0014-5793(98)01578-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A method for the purification of lysosomes from fibroblasts has been developed which uses endocytosis of superparamagnetic colloidal iron dextran particles followed by separation of the iron-containing lysosomes in a magnetic field. This permitted isolation of lysosomes from fibroblasts from patients with infantile sialic acid storage disorder and other lysosomal storage diseases in which a shift in lysosomal density induced by the storage material prevents purification by centrifugation in a Percoll gradient, The magnetic lysosomes isolated from these cells are very similar to those from normal cells as judged by lysosomal marker enzyme activity and 2D-PAGE analysis of the enriched proteins. (C) 1998 Federation of European Biochemical Societies.

引用

页码：369 / 372

页数：4

共 18 条

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