Poly(3-hydroxybutyrate) synthesis genes in Azotobacter sp strain FA8

被引：43

作者：

Pettinari, MJ

Vázquez, GJ

Silberschmidt, D

Rehm, B

Steinbüchel, A

Méndez, BS ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Biol, RA-1428 Buenos Aires, DF, Argentina

[2] Univ Munster, Inst Mikrobiol, D-48149 Munster, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 11期

关键词：

D O I：

10.1128/AEM.67.11.5331-5334.2001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FAS were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for beta -ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Ralstonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or octanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chain-length hydroxyalkanoates into PHB.

引用

页码：5331 / 5334

页数：4

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