The use of Armored RNA as a multi-purpose internal control for RT-PCR

被引:22
作者
Stevenson, Jeffery [1 ]
Hymas, Weston [1 ]
Hillyard, David [1 ,2 ]
机构
[1] Inst Clin & Expt Pathol, Salt Lake City, UT 84108 USA
[2] Univ Utah, Hlth Sci Ctr, Dept Pathol, Salt Lake City, UT 84112 USA
关键词
internal control; ms2; bacteriophage; RT-PCR; Armored RNA;
D O I
10.1016/j.jviromet.2008.02.007
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coli F-specific bacteriophage ms2, which is contained within the aRNA. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:73 / 76
页数:4
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