The use of Armored RNA as a multi-purpose internal control for RT-PCR
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作者:
Stevenson, Jeffery
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Inst Clin & Expt Pathol, Salt Lake City, UT 84108 USAInst Clin & Expt Pathol, Salt Lake City, UT 84108 USA
Stevenson, Jeffery
[1
]
Hymas, Weston
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Inst Clin & Expt Pathol, Salt Lake City, UT 84108 USAInst Clin & Expt Pathol, Salt Lake City, UT 84108 USA
Hymas, Weston
[1
]
Hillyard, David
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Inst Clin & Expt Pathol, Salt Lake City, UT 84108 USA
Univ Utah, Hlth Sci Ctr, Dept Pathol, Salt Lake City, UT 84112 USAInst Clin & Expt Pathol, Salt Lake City, UT 84108 USA
Hillyard, David
[1
,2
]
机构:
[1] Inst Clin & Expt Pathol, Salt Lake City, UT 84108 USA
[2] Univ Utah, Hlth Sci Ctr, Dept Pathol, Salt Lake City, UT 84112 USA
Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coli F-specific bacteriophage ms2, which is contained within the aRNA. (C) 2008 Elsevier B.V. All rights reserved.
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British Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, CanadaBritish Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, Canada
Eisler, DL
;
McNabb, A
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British Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, CanadaBritish Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, Canada
McNabb, A
;
Jorgensen, DR
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British Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, CanadaBritish Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, Canada
Jorgensen, DR
;
Isaac-Renton, JL
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British Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, CanadaBritish Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, Canada
机构:
Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Espy, MJ
;
Uhl, JR
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Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Uhl, JR
;
Sloan, LM
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Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Sloan, LM
;
Buckwalter, SP
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Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Buckwalter, SP
;
Jones, MF
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Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Jones, MF
;
Vetter, EA
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Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Vetter, EA
;
Yao, JDC
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Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Yao, JDC
;
Wengenack, NL
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Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Wengenack, NL
;
Rosenblatt, JE
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Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Rosenblatt, JE
;
Cockerill, FR
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Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Cockerill, FR
;
Smith, TF
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Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
机构:
British Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, CanadaBritish Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, Canada
Eisler, DL
;
McNabb, A
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British Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, CanadaBritish Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, Canada
McNabb, A
;
Jorgensen, DR
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British Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, CanadaBritish Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, Canada
Jorgensen, DR
;
Isaac-Renton, JL
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British Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, CanadaBritish Columbia Ctr Dis Control, Div Lab Serv, Mol Serv Lab, Vancouver, BC V5Z 4R4, Canada
机构:
Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Espy, MJ
;
Uhl, JR
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机构:
Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Uhl, JR
;
Sloan, LM
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机构:
Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Sloan, LM
;
Buckwalter, SP
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机构:
Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Buckwalter, SP
;
Jones, MF
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机构:
Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Jones, MF
;
Vetter, EA
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机构:
Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Vetter, EA
;
Yao, JDC
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机构:
Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Yao, JDC
;
Wengenack, NL
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机构:
Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Wengenack, NL
;
Rosenblatt, JE
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机构:
Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Rosenblatt, JE
;
Cockerill, FR
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机构:
Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA
Cockerill, FR
;
Smith, TF
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h-index: 0
机构:
Mayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USAMayo Clin & Mayo Fdn, Div Clin Microbiol, Dept Lab Med & Pathol, Rochester, MN 55905 USA