Pretreatment and Treatment With L-Arginine Attenuate Weight Loss and Bacterial Translocation in Dextran Sulfate Sodium Colitis

被引:43
作者
Rabelo Andrade, Maria Emilia [1 ]
Carvalho dos Santos, Rosana das Gracas [1 ]
Nascimento Soares, Anne Danieli [1 ]
Costa, Katia Anunciacao [1 ]
Antunes Fernandes, Simone Odilia [2 ]
de Souza, Cristina Maria [3 ]
Cassali, Geovanni Dantas [3 ]
de Souza, Adna Luciana [4 ]
Caetano Faria, Ana Maria [4 ]
Cardoso, Valbert Nascimento [2 ]
机构
[1] Univ Fed Minas Gerais, Fac Farm, Dept Alimentos, Belo Horizonte, MG, Brazil
[2] Univ Fed Minas Gerais, Fac Farm, Dept Anal Clin & Toxicol, Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Patol Geral, Belo Horizonte, MG, Brazil
[4] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Belo Horizonte, MG, Brazil
关键词
arginine; inflammatory bowel diseases; colitis; bacterial translocation; intestinal mucosa; NITRIC-OXIDE; INTESTINAL PERMEABILITY; LUMINAL ARGININE; BARRIER FUNCTION; MOUSE MODELS; GLUTAMINE; SUPPLEMENTATION; METABOLISM; INDUCTION; NUTRITION;
D O I
10.1177/0148607115581374
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 [营养与食品卫生学];
摘要
Background: Imbalances in a variety of factors, including genetics, intestinal flora, and mucosal immunity, can contribute to the development of ulcerative colitis and its side effects. This study evaluated the effects of pretreatment or treatment with arginine by oral administration on intestinal permeability, bacterial translocation (BT), and mucosal intestinal damage due to colitis. Methods: C57BL/6 mice were distributed into 4 groups: standard diet and water (C: control group), standard diet and dextran sodium sulfate (DSS) solution (Col: colitis group), 2% L-arginine supplementation for 7 days prior to DSS administration and during disease induction (PT: pretreated group), and 2% L-arginine supplementation during disease induction (T: treated group). Colitis was induced by administration of 1.5% DSS for 7 days. After 14 days, intestinal permeability and BT were evaluated; colons were collected for histologic analysis and determination of cytokines; feces were collected for measurement of immunoglobulin A (IgA). Results: The Col group showed increased intestinal permeability (C vs Col: P < .05) and BT (C vs Col: P < .05). In the arginine-supplemented groups (PT and T), this amino acid tended to decrease intestinal permeability. Arginine decreased BT to liver during PT (P < .05) and to blood, liver, spleen, and lung during T (P < .05). Histologic analysis showed that arginine preserved the intestinal mucosa and tended to decreased inflammation. Conclusions: Arginine attenuates weight loss and BT in mice with colitis.
引用
收藏
页码:1131 / 1139
页数:9
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