Influence of creatine supplementation on bone quality in the ovariectomized rat model: an FT-Raman spectroscopy study

被引:24
作者
de Souza, Renato Aparecido [1 ,4 ]
Xavier, Murilo [2 ,3 ]
da Silva, Fabiano Fernandes [1 ]
de Souza, Marco Tulio
Tosato, Maira Gaspar [5 ]
Martin, Airton Abrahao [5 ]
de Melo Castilho, Julio Cezar [6 ]
Ribeiro, Wellington [7 ]
Silveira, Landulfo, Jr. [3 ]
机构
[1] Inst Fed Educ Ciencia & Tecnol Sul Minas Gerais, Grp Estudos & Pesquisa Ciencias Saude, Muzambinho, MG, Brazil
[2] Vales Jequitinhonha & Mucuri Fed Univ, Dept Phys Therapy, Diamantina, MG, Brazil
[3] Univ Camilo Castelo Branco, Ctr Biomed Engn, Sao Jose Dos Campos, Brazil
[4] Vale do Sapucai Univ, Fac Med, Pouso Alegre, MG, Brazil
[5] Vale do Paraiba Univ, Inst Res & Dev, Lab Biomed Vibrat Spect, Sao Jose Dos Campos, Brazil
[6] Univ Estadual Paulista, Dept Radiol, Sao Jose Dos Campos, SP, Brazil
[7] Vale do Paraiba Univ, Inst Res & Dev, Lab Physiol & Pharmacodynam, Sao Jose Dos Campos, Brazil
关键词
Cortical bone; Nutritional supplement; Optical spectroscopy; Osteoporosis; Trabecular bone; AGE-RELATED-CHANGES; SKELETAL-MUSCLE; CORTICAL BONE; MONOHYDRATE; DENSITY; OSTEOPOROSIS; PERFORMANCE; INCREASES; PROSPECTS; STRENGTH;
D O I
10.1007/s10103-011-0976-0
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
The influence of creatine (Cr) supplementation on cortical and trabecular bone from ovariectomized rats was studied using FT-Raman spectroscopy. The intensity of organic-phase Raman bands was compared to mineral phase ones. Twenty-one female Wistar rats aged 3 months were divided into three groups (n = 7 per group): ovariectomized (OVX), ovariectomized treated with creatine (CRE) and sham-operated (SHAM) groups. Creatine supplementation (300 mg kg(-1) day(-1)) was provided for 8 weeks, starting 12 weeks after ovariectomy. FT-Raman spectroscopy was performed on the right medial femoral mid-shaft (cortical bone) and third lumbar vertebral body (trabecular bone). The integrated intensities of mineral phase (phosphate and carbonate bands at 959 and 1,071 cm(-1), respectively) and organic phase (amide I band at 1,665 cm(-1)) Raman bands were analyzed. The mineral-to-matrix (phosphate/amide I), carbonate-to-phosphate, and carbonate-to-amide I ratios were analyzed to assess bone quality. The phosphate content on trabecular bone was higher in the CRE group than the OVX group (p < 0.05). No significant changes in mineral or organic phases on cortical bone were observed. A radiographic assessment of bone density was encouraging as the same findings were showed by Raman intensity of phosphate from cortical (r (2) = 0.8037) and trabecular bones (r (2) = 0.915). Severe ovariectomy-induced bone loss was confirmed by FT-Raman spectroscopy. The results suggest that the chemical composition of trabecular bone tissue may be positively influenced by Cr supplementation after ovariectomy.
引用
收藏
页码:487 / 495
页数:9
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