RANKL Increases Migration of Human Lung Cancer Cells Through Intercellular Adhesion Molecule-1 Up-Regulation

被引:79
作者
Chen, Li-Mien [1 ,2 ]
Kuo, Chia-Hua [3 ]
Lai, Tung-Yuan [4 ,5 ]
Lin, Yueh-Min [6 ]
Su, Cheng-Chuan [7 ,8 ,9 ]
Hsu, His-Hsien [10 ]
Tsai, Fuu-Jen [5 ]
Tsai, Chang-Hai [11 ]
Huang, Chih-Yang [5 ,12 ,13 ]
Tang, Chih-Hsin [12 ,14 ]
机构
[1] Cent Taiwan Univ Sci & Technol, Ctr Gen Educ, Taichung, Taiwan
[2] Armed Force Taichung Gen Hosp, Dept Internal Med, Taichung, Taiwan
[3] Taipei Phys Educ Coll, Lab Exercise Biochem, Taipei, Taiwan
[4] China Med Univ, Sch Post Baccalaureate Chinese Med, Coll Chinese Med, Taichung, Taiwan
[5] China Med Univ Hosp, Dept Chinese Med, Taichung, Taiwan
[6] Changhua Christian Hosp, Dept Pathol, Changhua, Taiwan
[7] Buddhist Dalin Tzu Chi Gen Hosp, Dept Clin Pathol, Chiayi, Taiwan
[8] Buddhist Dalin Tzu Chi Gen Hosp, Dept Anat Pathol, Chiayi, Taiwan
[9] Tzu Chi Univ, Sch Med, Dept Pathol, Hualien, Taiwan
[10] Mackay Mem Hosp, Div Colorectal Surg, Taipei, Taiwan
[11] Asia Univ, Dept Healthcare Adm, Taichung, Taiwan
[12] China Med Univ & Hosp, Grad Inst Basic Med Sci, Taichung, Taiwan
[13] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung, Taiwan
[14] China Med Univ & Hosp, Sch Med, Dept Pharmacol, Taichung, Taiwan
关键词
RANKL; RANK; MIGRATION; LUNG CANCER; ERK; NF-KAPPA-B; HUMAN CHONDROSARCOMA CELLS; RECEPTOR ACTIVATOR; PROSTATE-CANCER; PATHWAY; EXPRESSION; METASTASIS; KINASE; DOMAIN; TUMOR;
D O I
10.1002/jcb.23009
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
Invasion of distant tissues by tumor cells is the primary cause of therapeutic failure in the treatment of malignant lung cancer cells. Receptor activator of nuclear factor-kappa B ligand (RANKL) and its receptor, RANK, play a key role in osteoclastogenesis and tumor metastasis. Intercellular adhesion molecule-1 (1CAM-1, also called CD54), a member of the immunoglobulin supergene family, is an inducible surface glycoprotein that mediates adhesion-dependent cell-to-cell interactions. The effects of RANKL on cell migration and ICAM-1 expression in human lung cancer cells are largely unknown. We found that RANKL directed the migration and increased ICAM-1 expression in human lung cancer (A549) cells. Pretreatment of A549 cells with the MAPK kinase (MEK) inhibitor PD98059 or U0126 inhibited RANKL-mediated migration and ICAM-1 expression. Stimulation of cells with RANKL increased the phosphorylation of MEK and extracellular signal-regulating kinase (ERK). In addition, an NF-kappa B inhibitor (PDTC) and I kappa B protease inhibitor (TPCK) also inhibited RANKL-mediated cell migration and ICAM-1 upregulation. Taken together, these results suggest that the RANKL and RANK interaction acts through MEK/ERK, which in turn activates NF-kappa B, resulting in the activation of ICAM-I and contributing to the migration of human lung cancer cells. J. Cell. Biochem. 112: 933-941, 2011. (C) 2010 Wiley-Liss, Inc.
引用
收藏
页码:933 / 941
页数:9
相关论文
共 33 条
[1]
RANKL acts directly on RANK-expressing prostate tumor cells and mediates migration and expression of tumor, metastasis genes [J].
Armstrong, Allison P. ;
Miller, Robert E. ;
Jones, Jon C. ;
Zhang, Jian ;
Keller, Evan T. ;
Dougall, William C. .
PROSTATE, 2008, 68 (01) :92-104
[2]
Osteoclast differentiation and activation [J].
Boyle, WJ ;
Simonet, WS ;
Lacey, DL .
NATURE, 2003, 423 (6937) :337-342
[3]
Expression of RANKL/RANK/OPG in primary and metastatic human prostate cancer as markers of disease stage and functional regulation [J].
Chen, Gaoping ;
Sircar, Kanishka ;
Aprikian, Armen ;
Potti, Anil ;
Goltzman, David ;
Rabbani, Shafaat A. .
CANCER, 2006, 107 (02) :289-298
[4]
CTGF enhances the motility of breast cancer cells via an integrin-αvβ3-ERK1/2-dependent S100A4-upregulated pathway [J].
Chen, Pai-Sheng ;
Wang, Ming-Yang ;
Wu, Shin-Ni ;
Su, Jen-Liang ;
Hong, Chih-Chen ;
Chuang, Shuang-En ;
Chen, Min-Wei ;
Hua, Kuo-Tai ;
Wu, Yu-Ling ;
Cha, Shih-Ting ;
Babu, Munisamy Suresh ;
Chen, Chiung-Nien ;
Lee, Po-Huang ;
Chang, King-Jen ;
Kuo, Min-Liang .
JOURNAL OF CELL SCIENCE, 2007, 120 (12) :2053-2065
[5]
Characterization of the intracellular domain of receptor activator of NF-κB (RANK) -: Interaction with tumor necrosis factor receptor-associated factors and activation of NF-κB and c-Jun N-terminal kinase [J].
Darnay, BG ;
Haridas, V ;
Ni, J ;
Moore, PA ;
Aggarwal, BB .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (32) :20551-20555
[6]
Activation of NF-κB by RANK requires tumor necrosis factor receptor-associated factor (TRAF) 6 and NF-κB-inducing kinase -: Identification of a novel TRAF6 interaction motif [J].
Darnay, BG ;
Ni, J ;
Moore, PA ;
Aggarwal, BB .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (12) :7724-7731
[7]
Integrins in cancer: biological implications and therapeutic opportunities [J].
Desgrosellier, Jay S. ;
Cheresh, David A. .
NATURE REVIEWS CANCER, 2010, 10 (01) :9-22
[8]
Duperray A, 1997, J BIOL CHEM, V272, P435
[9]
BMP-2 Increases Migration of Human Chondrosarcoma Cells Via PI3K/Akt Pathway [J].
Fong, Yi-Chin ;
Li, Te-Mao ;
Wu, Chi-Ming ;
Hsu, Sheng-Feng ;
Kao, Shung-Te ;
Chen, Ray-Jade ;
Lin, Cheng-Chieh ;
Liu, Shan-Chi ;
Wu, Chien-Lin ;
Tang, Chih-Hsin .
JOURNAL OF CELLULAR PHYSIOLOGY, 2008, 217 (03) :846-855
[10]
Cancer statistics, 2001 [J].
Greenlee, RT ;
Hill-Harmon, MB ;
Murray, T ;
Thun, M .
CA-A CANCER JOURNAL FOR CLINICIANS, 2001, 51 (01) :15-36