Microsatellite markers for Hebeloma species developed from expressed sequence tags in the ectomycorrhizal fungus Hebeloma cylindrosporum

被引:10
作者
Jany, JL [1 ]
Bousquet, J
Khasa, DP
机构
[1] Univ Laval, Chaire Rech Canada Genom Forestiere & Environm, Ste Foy, PQ G1K 7P4, Canada
[2] Univ Laval, Ctr Rech Biol Forestiere, Ste Foy, PQ G1K 7P4, Canada
来源
MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 04期
关键词
ectomycorrhizal fungus; expressed sequence tags; Hebeloma cylindrosporum; microsatellite;
D O I
10.1046/j.1471-8286.2003.00537.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The increasing number of expressed sequence tag (EST) projects dedicated to ectomycorrhizal fungi is translating into the release of large sets of ESTs. The aim of this study was to develop and test simple sequence repeat (SSR) markers from EST databases of the model ectomycorrhizal fungus Hebeloma cylindrosporum. Six SSR markers were found to be both unambiguously scorable and polymorphic among 12 H. cylindrosporum isolates. Two SSR markers were transferable to other Hebeloma species and one marker was interestingly found to be polymorphic among seven H. crustuliniforme isolates.
引用
收藏
页码:659 / 661
页数:3
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