In vivo on-line HPLC-microdialysis: simultaneous detection of monoamines and their metabolites in awake freely-moving rats

被引:36
作者
Chaurasia, CS [1 ]
Chen, CE
Ashby, CR
机构
[1] St Johns Univ, Coll Pharm & Allied Hlth Profess, Dept Pharm & Adm Sci, Jamaica, NY 11439 USA
[2] St Johns Univ, Coll Pharm & Allied Hlth Profess, Dept Pharmaceut Sci, Jamaica, NY 11439 USA
关键词
in vivo microdialysis; electrochemical detection; microbore column; dopamine; serotonin; metabolites; rat striatum; freely moving animal;
D O I
10.1016/S0731-7085(98)00182-4
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An on-line microbore high performance liquid chromatographic-electrochemical (HPLC-EC) detection method has been developed for the simultaneous measurement of dopamine (3,4-dihydroxyphenethylamine, DA), its metabolites 3,4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (3-methoxy-4-hydroxyphenylacetic acid, HVA), as well as serotonin (5-hydroxytryptamine, 5-HT) and its metabolise 5-hydroxyindole-3-acetic acid (5-HIAA) in the striatum of awake and freely moving rats. Our method was capable of detecting DOPAC, 5-HIAA, HVA, DA and 5-HT at retention time of 3, 5, 6.5, 9, and 24 min, respectively, in repeated on-line microdialysis sampling. Analysis was performed using a 150 x 1 mm 5 mu m Cls microbore column attached directly to a thin-layer radial flow electrochemical cell (UniJet(R)) comprising a 6 mm glassy carbon working electrode. In order to accommodate signal outputs (due to a varying level in the neurochemicals under investigation), an amperometric detector was equipped with a sensitivity programmable controlling software for automatically switching sensitivity inattentively and repeatedly for each collection cycle. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:413 / 422
页数:10
相关论文
共 25 条
[1]  
BOHS CE, 1994, CURR 0912, P181
[2]   EFFECT OF ACUTE MONOAMINE DEPLETION ON 3,4-METHYLENEDIOXYMETHAMPHETAMINE-INDUCED NEUROTOXICITY [J].
BRODKIN, J ;
MALYALA, A ;
NASH, JF .
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 1993, 45 (03) :647-653
[3]  
CHAPIN DS, 1986, CURRENT SEP, V7, P68
[4]   New dual electrochemical detector for microbore liquid chromatography - Determination of dopamine and serotonin in rat striatum dialysates [J].
Cheng, FC ;
Shih, Y ;
Liang, YJ ;
Yang, LL ;
Yang, CS .
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1996, 682 (02) :195-200
[5]   RAPID SAMPLING AND DETERMINATION OF EXTRACELLULAR DOPAMINE INVIVO [J].
CHURCH, WH ;
JUSTICE, JB .
ANALYTICAL CHEMISTRY, 1987, 59 (05) :712-716
[6]  
Dewey SL, 1997, SYNAPSE, V25, P393, DOI 10.1002/(SICI)1098-2396(199704)25:4<393::AID-SYN11>3.3.CO
[7]  
2-S
[8]  
HUANG T, 1994, CURR 0912, P191
[9]  
HUANG T, 1990, CURR SEP, V10, P16
[10]  
HUANG T, 1990, CURRENT SEP, V9, P139