A transgenic zebrafish model of neutrophilic inflammation

被引：754

作者：

Renshaw, Stephen A.

Loynes, Catherine A.

Trushell, Daniel M. I.

Elworthy, Stone

Ingham, Philip W.

Whyte, Moira K. B.

机构：

[1] Univ Sheffield, Sch Med & Biomed Sci, Acad Unit Resp Med, MRC Ctr Dev & Biomed Genet, Sheffield, S Yorkshire, England

[2] Univ Sheffield, Dept Biomed Sci, Sheffield, S Yorkshire, England

来源：

BLOOD | 2006年 / 108卷 / 13期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1182/blood-2006-05-024075

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have established an in vivo model for genetic analysis of the inflammatory response by generating a transgenic zebrafish line that expresses GFP under the neutrophil-specific myeloperoxidase promoter. We show that inflammation is induced after transection of the tail of zebrafish larvae and that this inflammation subsequently resolves over a similar time course to mammalian systems. Quantitative data can be generated from this model by counting of fluorescent cells or by digital image analysis. In addition, we show that the resolution of experimentally induced inflammation can be inhibited by the addition of a pancaspase inhibitor, zVD.fmk, demonstrating that experimental manipulation of the resolution of inflammation is possible in this model,

引用

页码：3976 / 3978

页数：3

共 15 条

[1]

[Anonymous], 2004, IMAGEJ COMPUTER PROG

[2]

AUSTIN GE, 1995, LEUKEMIA, V9, P848

[3] Myelopoiesis in the zebrafish, Danio rerio [J].

Bennett, CM ;

Kanki, JP ;

Rhodes, J ;

Liu, TX ;

Paw, BH ;

Kieran, MW ;

Langenau, DM ;

Delahaye-Brown, A ;

Zon, LI ;

Fleming, MD ;

Look, AT .

BLOOD, 2001, 98 (03) :643-651

[4] RESOLUTION OF ACUTE-INFLAMMATION AND THE ROLE OF APOPTOSIS IN THE TISSUE FATE OF GRANULOCYTES [J].

HASLETT, C .

CLINICAL SCIENCE, 1992, 83 (06) :639-648

[5] The pu. 1 promoter drives myeloid gene expression in zebrafish [J].

Hsu, K ;

Traver, D ;

Kutok, JL ;

Hagen, A ;

Liu, TX ;

Paw, BH ;

Rhodes, J ;

Berman, JN ;

Zon, LI ;

Kanki, JP ;

Look, AT .

BLOOD, 2004, 104 (05) :1291-1297

[6] A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA [J].

Lee, EC ;

Yu, DG ;

de Velasco, JM ;

Tessarollo, L ;

Swing, DA ;

Court, DL ;

Jenkins, NA ;

Copeland, NG .

GENOMICS, 2001, 73 (01) :56-65

[7] Morphologic and functional characterization of granulocytes and macrophages in embryonic and adult zebrafish [J].

Lieschke, GJ ;

Oates, AC ;

Crowhurst, MO ;

Ward, AC ;

Layton, JE .

BLOOD, 2001, 98 (10) :3087-3096

[8] Granulocyte colony-stimulating factor inhibits the mitochondria-dependent activation of caspase-3 in neutrophils [J].

Maianski, NA ;

Mul, FRJ ;

van Buul, JD ;

Roos, D ;

Kuijpers, TW .

BLOOD, 2002, 99 (02) :672-679

[9]

Nusslein-Volhard C., 2002, ZEBRAFISH PRACTICAL

[10] Imaging macrophage chemotaxis in vivo: Studies of microtubule function in zebrafish wound inflammation [J].

Redd, Michael J. ;

Kelly, Gavin ;

Dunn, Graham ;

Way, Michael ;

Martin, Paul .

CELL MOTILITY AND THE CYTOSKELETON, 2006, 63 (07) :415-422

← 1 2 →