Orthotopic replacement of the aortic valve with decellularized allograft in a sheep model

被引:96
作者
Baraki, Hassina [1 ]
Tudorache, Igor [1 ]
Braun, Maike [1 ]
Hoeffler, Klaus [1 ]
Goerler, Adelheid [1 ]
Lichtenberg, Artur [1 ]
Bara, Christopher [1 ]
Calistru, Alex [1 ]
Brandes, Gudrun [2 ]
Hewicker-Trautwein, Marion [3 ]
Hilfiker, Andres [1 ]
Haverich, Axel [1 ]
Cebotari, Serghei [1 ]
机构
[1] Hannover Med Sch, Dept Cardiothorac Transplantat & Vasc Surg, D-30625 Hannover, Germany
[2] Hannover Med Sch, Dept Cell Biol, D-30625 Hannover, Germany
[3] Univ Vet Med, Dept Pathol, Hannover, Germany
关键词
Aorta; Valves; Surgery; Tissue engineering; HEART-VALVE; TISSUE; CELLS;
D O I
10.1016/j.biomaterials.2009.07.068
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Tissue engineered (TE) allografts have been successfully applied in pulmonary circulation. The behavior of TE valves based on decellularized scaffolds in systemic circulation remains unexplored. We investigated the function, histological changes, potential of in-vivo re-endothelialization of decellularized aortic valve allografts in orthotopic position in sheep. Ovine aortic valve conduits (n = 12) were decellularized with detergents and implanted as an aortic root in lambs (35-45 kg). For controls, fresh native ovine aortic valve conduits (n = 6) were implanted. The valves were explanted at 3 and 9 months. In the experimental group, the valves exhibited trivial regurgitation and normal morphology with no signs of graft dilatation, degeneration or rejection. In some animals (n = 2), we documented minimal calcification in the area of arterial anastomosis and in one, microthrombi formation on the leaflet surface. The luminal sides of the grafts were partially covered with an endothelial cell monolayer, neovasculogenesis was observed at the adventitial side. The valves in the control group appeared thickened, shrunken with marked calcification/degeneration signs, and advanced valve insufficiency. Detergent decellularized aortic valve allografts satisfy the higher requirements of the systemic circulation in sheep. As valve conduits become repopulated by endothelial and interstitial cells, they may re-gain the potential for growth. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:6240 / 6246
页数:7
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