Application of mitochondrial control region in population genetic studies of the shrimp Penaeus

被引:51
作者
Chu, KH [1 ]
Li, CP
Tam, YK
Lavery, S
机构
[1] Chinese Univ Hong Kong, Dept Biol, Shatin, Hong Kong, Peoples R China
[2] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA
来源
MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 01期
关键词
control region; mitochondrial DNA; penaeid shrimp; Penaeus; Penaeus merguiensis; population genetics;
D O I
10.1046/j.1471-8286.2003.00376.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study reports a primer set for amplifying a partial fragment of about 610 bp in the fast mutating mitochondrial control region in shrimps of the genus Penaeus (Decapoda: Penaeidae). The utility of this amplified fragment for studying population differentiation and structuring, compared with more conservative mitochondrial genes (16S rRNA and COI), was explored in P. merguiensis populations over a vast geographical range based on sequence and RFLP analyses. The results indicate that the mitochondrial control region provides more informative sites and reveals more haplotypes, making it most useful for evaluating genetic variations within and between populations of Penaeus species.
引用
收藏
页码:120 / 122
页数:3
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