Ultrasound-assisted microbubbles gene transfer in tendons for gene therapy

被引：30

作者：

Delalande, Anthony ^{[1
,3
]}

Bureau, Michel-Francis ^{[2
]}

Midoux, Patrick ^{[1
]}

Bouakaz, Ayache ^{[3
]}

Pichon, Chantal ^{[1
]}

机构：

[1] CNRS, Ctr Biophys Mol, UPR 4301, F-45071 Orleans, France

[2] CNRS, Lab Pharmacol Chim & Genet, UMR 8151, F-75270 Paris, France

[3] Univ Tours, INSERM, CNRS, U930,ERL 3106, Tours, France

来源：

ULTRASONICS | 2010年 / 50卷 / 02期

关键词：

Ultrasound; Microbubbles; Tendon; Gene transfer; Gene therapy; CONTRAST AGENTS; PLASMID DNA; SKELETAL-MUSCLE; CULTURED-CELLS; CAROTID-ARTERY; IN-VITRO; SONOPORATION; DELIVERY; IMPROVES; REPAIR;

D O I：

10.1016/j.ultras.2009.09.035

中图分类号：

O42 [声学];

学科分类号：

070206 [声学];

摘要：

Our study aimed at evaluating the use of ultrasound-assisted microbubbles gene transfer in mice Achilles tendons. Using a plasmid encoding luciferase gene, it was found that an efficient and stable gene expression for more than two weeks was obtained when tendons were injected with 10 mu g of plasmid in the presence of 5 x 10(5) BR14 microbubbles with the following acoustic parameters: 1 MHz, 200 kPa, 40% duty cycle and 10 min of exposure time. The rate of gene expression was 100-fold higher than that obtained with naked plasmid injected alone without ultrasound or with ultrasound in absence of microbubbles. The long term expression of transgene makes ultrasound-assisted microbubble a suitable method for gene therapy in tendons. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：269 / 272

页数：4

共 23 条

[1]

Ultrasound-mediated delivery of TIMP-3 plasmid DNA into saphenous vein leads to increased lumen size in a porcine interposition graft model [J].