Simplified protocol to isolate, purify, and culture expand mesenchymal stem cells

被引：34

作者：

Schrepfer, Sonja

Deuse, Tobias

Lange, Claudia

Katzenberg, Regina

Reichenspurner, Hermann

Robbins, Robert C.

Pelletier, Marc P.

机构：

[1] Stanford Univ, Dept Cardiothorac Surg, Sch Med, Stanford, CA 94305 USA

[2] Univ Hamburg, Dept Cardiovasc Surg, Ctr Heart, D-20246 Hamburg, Germany

[3] Univ Hamburg Hosp, Dept Bone Marrow Transplant, D-20246 Hamburg, Germany

[4] Stanford Univ, Dept Cardiovasc Med, Sch Med, Stanford, CA 94305 USA

来源：

STEM CELLS AND DEVELOPMENT | 2007年 / 16卷 / 01期

关键词：

D O I：

10.1089/scd.2006.0041

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Mesenchymal stem cells (MSCs) are widely used for experimental regenerative strategies. Due to their differentiation capacity into mesenchymal lineages, they are a potential cellular source for tissue regeneration. Because there is no specific antigen that can be used to define MSCs directly, there is no consensus about how to isolate them. Here we describe a simple protocol to isolate, purify, and culture expand murine bone marrow MSCs using magnetic cell sorting and plastic adherence. We further show that cytokine supplementation enhances MSC proliferation without jeopardizing their pluripotency.

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页码：105 / 107

页数：3

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