Model building of a molluscan hemocyanin from X-ray solution scattering

被引：3

作者：

Micetic, I ^{[1
]}

Salvato, B ^{[1
]}

机构：

[1] Univ Padua, Dept Biol, I-35131 Padua, Italy

来源：

MICRON | 2004年 / 35卷 / 1-2期

关键词：

hemocyanin; modeling; small angle X-ray scattering; cryoelectron microscopy; simulated annealing;

D O I：

10.1016/j.micron.2003.10.006

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Molluscan hemocyanins are proteins of a truly enormous size. Because of this, determination of their quaternary structure at high resolution cannot easily be obtained by standard methods such as X-ray crystallography and NMR. Therefore, different approaches, using several low-resolution techniques are currently necessary to understand hemocyanin structure. In this work a model of the Rapana venosa hemocyanin has been obtained from a template model and small angle X-ray scattering (SAXS) data. The template model was built from the electron density of the closely related Haliotis tuberculata hemocyanin and a computer program was written to fit this model to the SAXS data using the simulated annealing algorithm. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：17 / 20

页数：4

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