Dispersion and culture of magnocellular neurons from the supraoptic nucleus of the adult rat

被引：10

作者：

Bilinski, M ^{[1
]}

Sanchez, A ^{[1
]}

Nicolini, VG ^{[1
]}

Villar, MJ ^{[1
]}

Tramezzani, JH ^{[1
]}

机构：

[1] UNIV BUENOS AIRES,FAC CIENCIAS VET,BUENOS AIRES,DF,ARGENTINA

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1996年 / 64卷 / 01期

关键词：

hypothalamus; vasopressin; oxytocin; monolayer culture; immunohistochemistry; HYPOTHALAMO-NEUROHYPOPHYSEAL SYSTEM; NEUROENDOCRINE CELLS; VASOPRESSIN RELEASE; AREA;

D O I：

10.1016/0165-0270(95)00030-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and efficient procedure for the dispersion and culture of magnocellular neurons of the adult rat hypothalamus was developed. The enzymatically and mechanically dispersed cells were highly viable and showed neurite outgrowth after 3 days of culture. The neurons could be maintained for more than 4 weeks without any sign of deterioration. Light-optic immunocytochemistry of the cultured cells revealed the presence of many oxytocin- and vasopressin immunoreactive neurons. The results indicate that the method may be suitable for many experimental purposes.

引用

页码：13 / 18

页数：6

共 14 条

[1] CULTURED HYPOTHALAMIC EXPLANTS FROM SPONTANEOUSLY HYPERTENSIVE RATS HAVE DECREASED VASOPRESSIN AND OXYTOCIN CONTENT AND RELEASE [J].

BENNETT, BA ;

CLODFELTER, J ;

SUNDBERG, DK ;

MORRIS, M .

AMERICAN JOURNAL OF HYPERTENSION, 1989, 2 (01) :46-51

[2] WHOLE CELL VOLTAGE CLAMP RECORDINGS FROM CULTURED NEURONS OF THE SUPRAOPTIC AREA OF NEONATAL RAT HYPOTHALAMUS [J].

COBBETT, P ;

MASON, WT .

BRAIN RESEARCH, 1987, 409 (01) :175-180

[3] CHARACTERIZATION OF 3 TYPES OF POTASSIUM CURRENT IN CULTURED NEURONS OF RAT SUPRAOPTIC NUCLEUS AREA [J].

COBBETT, P ;

LEGENDRE, P ;

MASON, WT .

JOURNAL OF PHYSIOLOGY-LONDON, 1989, 410 :443-462

[4] A COMPARTMENTALIZED, ORGAN-CULTURED HYPOTHALAMO-NEUROHYPOPHYSEAL SYSTEM FOR THE STUDY OF VASOPRESSIN RELEASE [J].

GREGG, CM ;

SLADEK, CD .

NEUROENDOCRINOLOGY, 1984, 38 (05) :397-402

[5] ISOLATION OF NEURONS SUITABLE FOR PATCH-CLAMPING FROM ADULT MAMMALIAN CENTRAL NERVOUS SYSTEMS [J].

KAY, AR ;

WONG, RKS .

JOURNAL OF NEUROSCIENCE METHODS, 1986, 16 (03) :227-238

[6] PLATELETS AS A SOURCE OF FIBROBLAST GROWTH-PROMOTING ACTIVITY [J].

KOHLER, N ;

LIPTON, A .

EXPERIMENTAL CELL RESEARCH, 1974, 87 (02) :297-301

[7] PROPERTIES OF SUPRAOPTIC MAGNOCELLULAR NEURONS ISOLATED FROM THE ADULT-RAT [J].

OLIET, SHR ;

BOURQUE, CW .

JOURNAL OF PHYSIOLOGY-LONDON, 1992, 455 :291-306

[8]

SANCHEZ A, 1992, 5 IB C CELL BIOL MAL, P135

[9]

SHAHAR J, 1989, DISSECTION TISSUE CU

[10] CHOLINERGIC STIMULATION OF VASOPRESSIN RELEASE FROM RAT HYPOTHALAMO-NEUROHYPOPHYSEAL SYSTEM IN ORGAN-CULTURE [J].

SLADEK, CD ;

KNIGGE, KM .

ENDOCRINOLOGY, 1977, 101 (02) :411-420

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