Serine recombinases as tools for genome engineering

被引：76

作者：

Brown, William R. A. ^{[1
]}

Lee, Nicholas C. O. ^{[1
]}

Xu, Zhengyao ^{[1
]}

Smith, Margaret C. M. ^{[2
]}

机构：

[1] Queens Med Ctr, Sch Biol, Nottingham NG7 2UH, England

[2] Univ Aberdeen, Inst Med Sci, Aberdeen AB25 2ZD, Scotland

来源：

METHODS | 2011年 / 53卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Serine recombinases; phi C31 integrase; Unidirectional; Site-specific recombination; SITE-SPECIFIC RECOMBINATION; PHAGE PHI-C31 INTEGRASE; GAMMA-DELTA-RESOLVASE; MAMMALIAN-CELLS; DIRECTED EVOLUTION; SYSTEM; CRE; EXPRESSION; DIVERSITY; MECHANISM;

D O I：

10.1016/j.ymeth.2010.12.031

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The serine recombinases differ mechanistically from the tyrosine recombinases and include proteins such as phi C31 integrase which, unlike Cre and Flp, promote unidirectional reactions. The serine recombinase family is large and includes many other proteins besides phi C31 integrase with the potential to be widely used in genome engineering. Here we review the details of the mechanism of the reactions promoted by the serine recombinases and discuss how these not only limit the utility of this class of recombinase but also creates opportunities for the engineering of new enzymes. We discuss the unanswered questions posed by genome engineering experiments in a variety of systems in which the serine recombinases have been used and finally describe more recently discovered serine recombinases that have the potential to be used in genome engineering. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：372 / 379

页数：8

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