Effect of the protein kinase C inhibitor GF 109 203X on elastase release and respiratory burst of human neutrophils

被引：26

作者：

Cabanis, A ^{[1
]}

Gressier, B ^{[1
]}

Brunet, C ^{[1
]}

Dine, T ^{[1
]}

Luyckx, M ^{[1
]}

Cazin, M ^{[1
]}

Cazin, JC ^{[1
]}

机构：

[1] FAC SCI PHARMACEUT & BIOL,PHARMACOL LAB,PHARMACOCINET & PHARM CLIN,F-59006 LILLE,FRANCE

来源：

GENERAL PHARMACOLOGY | 1996年 / 27卷 / 08期

关键词：

human neutrophils; reactive oxygen species; elastase; proteins kinase C inhibitor;

D O I：

10.1016/S0306-3623(96)00053-5

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

1. The effects of bisindolylmaleimide GF 109 203X, reported to be a potent and highly selective inhibitor of protein kinase C (PKC), have been investigated on some human neutrophil functions. 2. GF 109 203X prevented O-2(-) production by NADPH-oxidase whatever the stimulus used for polymorphonuclear neutrophil (PMN) activation: directs PKC activators like phorbol myristate acetate (PMA) and dioctanoylglycerol, calcium ionophore (A23187), or receptor agonists like fMet-Leu-Phe (fMLP) and opsonized zymosan. 3. The effect of GF 109 203X was also examined on elastase exocytosis by neutrophils. PMA-mediated elastase release was prevented by GF 109 203X. However, GF 109 203X had no effect on exocytosis induced by A23187 and the effect of this compound on the fMLP response changed according to its concentration. 4. These data suggest that PKC might be essential for stimulus-mediated O-2(-) production and also that PKC plays only a minor role in elastase secretion as compared to the role of the cytosolic calcium level. Copyright (C) 1996 Elsevier Science Inc.

引用

页码：1409 / 1414

页数：6

共 40 条

[1] THE ANTIOXIDANT ACTION OF N-ACETYLCYSTEINE - ITS REACTION WITH HYDROGEN-PEROXIDE, HYDROXYL RADICAL, SUPEROXIDE, AND HYPOCHLOROUS ACID [J].

ARUOMA, OI ;

HALLIWELL, B ;

HOEY, BM ;

BUTLER, J .

FREE RADICAL BIOLOGY AND MEDICINE, 1989, 6 (06) :593-597

[2] ACTIVATION OF NEUTROPHIL LEUKOCYTES - CHEMOATTRACTANT RECEPTORS AND RESPIRATORY BURST [J].

BAGGIOLINI, M ;

BOULAY, F ;

BADWEY, JA ;

CURNUTTE, JT .

FASEB JOURNAL, 1993, 7 (11) :1004-1010

[3]

BIRCHALL AM, 1994, J PHARMACOL EXP THER, V268, P922

[4] PHOSPHOLIPASE-D ACTIVATION IS FUNCTIONALLY LINKED TO SUPEROXIDE GENERATION IN THE HUMAN NEUTROPHIL [J].

BONSER, RW ;

THOMPSON, NT ;

RANDALL, RW ;

GARLAND, LG .

BIOCHEMICAL JOURNAL, 1989, 264 (02) :617-620

[5] THERAPEUTIC POTENTIAL OF PROTEIN-KINASE-C INHIBITORS [J].

BRADSHAW, D ;

HILL, CH ;

NIXON, JS ;

WILKINSON, SE .

AGENTS AND ACTIONS, 1993, 38 (1-2) :135-147

[6] A RAPID DENSITY GRADIENT TECHNIQUE FOR SEPARATING POLYMORPHONUCLEAR GRANULOCYTES [J].

CABANIS, A ;

GRESSIER, B ;

LEBEGUE, S ;

BRUNET, C ;

DINE, T ;

LUYCKX, M ;

CAZIN, M ;

CAZIN, JC .

APMIS, 1994, 102 (02) :119-121

[7]

CABANIS A, 1993, PHARM PHARM LETT, V2, P236

[8] 2 CYTOSOLIC COMPONENTS OF THE HUMAN NEUTROPHIL RESPIRATORY BURST OXIDASE TRANSLOCATE TO THE PLASMA-MEMBRANE DURING CELL ACTIVATION [J].

CLARK, RA ;

VOLPP, BD ;

LEIDAL, KG ;

NAUSEEF, WM .

JOURNAL OF CLINICAL INVESTIGATION, 1990, 85 (03) :714-721

[9]

COCHRANE CG, 1991, AM J MED S3C, V91, P23

[10] SUPEROXIDE GENERATION BY DIGITONIN-STIMULATED GUINEA-PIG GRANULOCYTES - BASIS FOR A CONTINUOUS ASSAY FOR MONITORING SUPEROXIDE PRODUCTION AND FOR STUDY OF ACTIVATION OF GENERATING SYSTEM [J].

COHEN, HJ ;

CHOVANIEC, ME .

JOURNAL OF CLINICAL INVESTIGATION, 1978, 61 (04) :1081-1087

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