Fermentation of acid-pretreated corn stover to ethanol without detoxification using Pichia stipitis

被引：22

作者：

Agbogbo, Frank K. ^{[1
]}

Haagensen, Frank D. ^{[1
]}

Milam, David ^{[1
]}

Wenger, Kevin S. ^{[1
]}

机构：

[1] Novozymes N Amer, Franklinton, NC 27525 USA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2008年 / 145卷 / 1-3期

关键词：

corn stover; ethanol; sulfuric acid; pichia stipitis; adaptation; detoxification;

D O I：

10.1007/s12010-007-8056-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this work, the effect of adaptation on P. stipitis fermentation using acid-pretreated corn stover hydrolyzates without detoxification was examined. Two different types of adaptation were employed, liquid hydrolyzate and solid state agar adaptation. Fermentation of 12.5% total solids undetoxified acid-pretreated corn stover was performed in shake flasks at different rotation speeds. At low rotation speed (100 rpm), both liquid hydrolyzate and solid agar adaptation highly improved the sugar consumption rate as well as ethanol production rate compared to the wild-type strains. The fermentation rate was higher for solid agar-adapted strains compared to liquid hydrolyzate-adapted strains. At a higher rotation speed (150 rpm), there was a faster sugar consumption and ethanol production for both the liquid-adapted and the wild-type strains. However, improvements in the fermentation rate between the liquid-adapted and wild strains were less pronounced at the high rotation speed.

引用

页码：53 / 58

页数：6

共 19 条

[1] The effect of initial cell concentration on xylose fermentation by Pichia stipitis [J].