Imparting temperature sensitivity and attenuation in ferrets to A/Puerto Rico/8/34 influenza virus by transferring the genetic signature for temperature sensitivity from cold-adapted A/Ann Arbor/6/60

被引：71

作者：

Jin, H ^{[1
]}

Zhou, H ^{[1
]}

Lu, B ^{[1
]}

Kemble, G ^{[1
]}

机构：

[1] MedImmune Vaccines Inc, Mountain View, CA 94043 USA

来源：

JOURNAL OF VIROLOGY | 2004年 / 78卷 / 02期

关键词：

D O I：

10.1128/JVI.78.2.995-998.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The four temperature-sensitive (ts) loci identified in the PB1 and PB2 gene segments of cold-adapted A/Ann Arbor/6/60 influenza virus, the master donor virus for influenza A virus (MDV-A) FluMist vaccines, were introduced into a divergent A/Puerto Rico/8/34 influenza virus strain. Recombinant A/Puerto Rico/8/34 virus with these four introduced ts loci exhibited both ts and att phenotypes similar to those of MDV-A, which could be used as a donor virus for manufacturing large quantities of inactivated influenza virus vaccine against potential pandemic strains.

引用

页码：995 / 998

页数：4

共 19 条

[1] Previous H1N1 influenza A viruses circulating in the Mongolian population [J].