Spo11-Accessory Proteins Link Double-Strand Break Sites to the Chromosome Axis in Early Meiotic Recombination

被引：273

作者：

Panizza, Silvia ^{[1
]}

Mendoza, Marco A. ^{[1
]}

Berlinger, Marc ^{[1
]}

Huang, Lingzhi ^{[1
]}

Nicolas, Alain ^{[2
]}

Shirahige, Katsuhiko ^{[3
]}

Klein, Franz ^{[1
]}

机构：

[1] Univ Vienna, Dept Chromosome Biol, Max F Perutz Labs, A-1030 Vienna, Austria

[2] Univ Paris 06, CNRS, UMR3244, Inst Curie,Ctr Rech, F-75248 Paris 05, France

[3] Univ Tokyo, Inst Mol & Cellular Biosci, Res Ctr Epigenet Dis, Lab Genome Struct & Funct,Bunkyo Ku, Tokyo 1130032, Japan

来源：

CELL | 2011年 / 146卷 / 03期

基金：

奥地利科学基金会;

关键词：

ANAPHASE-PROMOTING COMPLEX; SACCHAROMYCES-CEREVISIAE; FUNCTIONAL INTERACTIONS; SYNAPTONEMAL COMPLEXES; CHIASMA FORMATION; DNA-REPLICATION; YEAST; INITIATION; MEIOSIS; SPO11;

D O I：

10.1016/j.cell.2011.07.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Meiotic recombination between homologous chromosomes initiates via programmed DNA double-strand breaks (DSBs), generated by complexes comprising Spo11 transesterase plus accessory proteins. DSBs arise concomitantly with the development of axial chromosome structures, where the coalescence of axis sites produces linear arrays of chromatin loops. Recombining DNA sequences map to loops, but are ultimately tethered to the underlying axis. How and when such tethering occurs is currently unclear. Using ChIPchip in yeast, we show that Spo11-accessory proteins Rec114, Mer2, and Mei4 stably interact with chromosome axis sequences, upon phosphorylation of Mer2 by S phase Cdk. This axis tethering requires meiotic axis components (Red1/Hop1) and is modulated in a domain-specific fashion by cohesin. Loss of Rec114, Mer2, and Mei4 binding correlates with loss of DSBs. Our results strongly suggest that hot-spot sequences become tethered to axis sites by the DSB machinery prior to DSB formation.

引用

页码：372 / 383

页数：12

共 68 条

[1] RecA-like proteins are components of early meiotic nodules in lily [J].