Tol2: a versatile gene transfer vector in vertebrates

被引：370

作者：

Kawakami, Koichi ^{[1
,2
]}

机构：

[1] Grad Univ Adv Studies SOKENDAI, Natl Inst Genet, Div Mol & Dev Biol, Mishima, Shizuoka 4118540, Japan

[2] Grad Univ Adv Studies SOKENDAI, Dept Genet, Mishima, Shizuoka 4118540, Japan

来源：

GENOME BIOLOGY | 2007年 / 8卷

关键词：

D O I：

10.1186/gb-2007-8-S1-S7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The medaka fish Tol2 element is an autonomous transposon that encodes a fully functional transposase. The transposase protein can catalyze transposition of a transposon construct that has 200 and 150 base pairs of DNA from the left and right ends of the Tol2 sequence, respectively. These sequences contain essential terminal inverted repeats and subterminal sequences. DNA inserts of fairly large sizes (as large as 11 kilobases) can be cloned between these sequences without reducing transpositional activity. The Tol2 transposon system has been shown to be active in all vertebrate cells tested thus far, including zebrafish, Xenopus, chicken, mouse, and human. In this review I describe and discuss how the Tol2 transposon is being applied to transgenic studies in these vertebrates, and possible future applications.

引用

收藏

页数：10

相关论文

共 46 条

[1] THE AEQUOREA-VICTORIA GREEN FLUORESCENT PROTEIN CAN BE USED AS A REPORTER IN LIVE ZEBRAFISH EMBRYOS [J].

AMSTERDAM, A ;

LIN, S ;

HOPKINS, N .

DEVELOPMENTAL BIOLOGY, 1995, 171 (01) :123-129

[2] Harnessing a high cargo-capacity transposon for genetic applications in vertebrates [J].

Balciunas, Darius ;

Wangensteen, Kirk J. ;

Wilber, Andrew ;

Bell, Jason ;

Geurts, Aron ;

Sivasubbu, Sridhar ;

Wang, Xin ;

Hackett, Perry B. ;

Largaespada, David A. ;

McIvor, R. Scott ;

Ekker, Stephen C. .

PLOS GENETICS, 2006, 2 (11) :1715-1724

[3] Migration of zebrafish primordial germ cells: A role for myosin contraction and cytoplasmic flow [J].

Blaser, Heiko ;

Reichman-Fried, Michal ;

Castanon, Irinka ;

Dumstrei, Karin ;

Marlow, Florence L. ;

Kawakami, Koichi ;

Solnica-Krezel, Lilianna ;

Heisenberg, Carl-Philipp ;

Raz, Erez .

DEVELOPMENTAL CELL, 2006, 11 (05) :613-627

[4] GERMLINE TRANSMISSION OF EXOGENOUS GENES IN THE CHICKEN [J].

BOSSELMAN, RA ;

HSU, RY ;

BOGGS, T ;

HU, S ;

BRUSZEWSKI, J ;

OU, S ;

KOZAR, L ;

MARTIN, F ;

GREEN, C ;

JACOBSEN, F ;

NICOLSON, M ;

SCHULTZ, JA ;

SEMON, KM ;

RISHELL, W ;

STEWART, RG .

SCIENCE, 1989, 243 (4890) :533-535

[5] TRANSPOSON MUTAGENESIS OF BACULOVIRUSES - ANALYSIS OF TRICHOPLUSIA-NI TRANSPOSON IFP2 INSERTIONS WITHIN THE FP-LOCUS OF NUCLEAR POLYHEDROSIS VIRUSES [J].

CARY, LC ;

GOEBEL, M ;

CORSARO, BG ;

WANG, HG ;

ROSEN, E ;

FRASER, MJ .

VIROLOGY, 1989, 172 (01) :156-169

[6] Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system [J].

Dupuy, AJ ;

Akagi, K ;

Largaespada, DA ;

Copeland, NG ;

Jenkins, NA .

NATURE, 2005, 436 (7048) :221-226

[7] Transposition and gene disruption in the male germline of the mouse [J].

Dupuy, AJ ;

Fritz, S ;

Largaespada, DA .

GENESIS, 2001, 30 (02) :82-88

[8] Regulated transposition of a fish transposon in the mouse germ line [J].

Fischer, SEJ ;

Wienholds, E ;

Plasterk, RHA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (12) :6759-6764

[9] Conservation of RET regulatory function from human to zebrafish without sequence similarity [J].

Fisher, S ;

Grice, EA ;

Vinton, RM ;

Bessling, SL ;

McCallion, AS .

SCIENCE, 2006, 312 (5771) :276-279

[10] Evaluating the biological relevance of putative enhancers using Tol2 transposon-mediated transgenesis in zebrafish [J].

Fisher, Shannon ;

Grice, Elizabeth A. ;

Vinton, Ryan M. ;

Bessling, Seneca L. ;

Urasaki, Akihiro ;

Kawakami, Koichi ;

McCallion, Andrew S. .

NATURE PROTOCOLS, 2006, 1 (03) :1297-1305

← 1 2 3 4 5 →