Thioflavin T forms a non-fluorescent complex with α-helical poly-L-glutamic acid

被引：26

作者：

Babenko, Viktoria ^{[1
]}

Dzwolak, Wojciech ^{[1
]}

机构：

[1] Univ Warsaw, Dept Chem, PL-02093 Warsaw, Poland

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 38期

关键词：

AMYLOID FIBRILS; CIRCULAR-DICHROISM; BINDING; INSULIN; SUPERSTRUCTURES; MICROSCOPY; ASSEMBLIES; DYE;

D O I：

10.1039/c1cc14230e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Thioflavin T (ThT) is a molecular-rotor-type fluorophore reputed for the selective binding to amyloid fibrils. Using induced circular dichroism, here we show that ThT binds in an orderly manner to alpha-helical poly-L-glutamic acid (PLGA) implying that neither stacked beta-sheets nor pi-pi stacking interactions are necessary for the binding between the dye and proteins.

引用

收藏

页码：10686 / 10688

页数：3

相关论文

共 16 条

[1] Amyloid-binding compounds maintain protein homeostasis during ageing and extend lifespan [J].

Alavez, Silvestre ;

Vantipalli, Maithili C. ;

Zucker, David J. S. ;

Klang, Ida M. ;

Lithgow, Gordon J. .

NATURE, 2011, 472 (7342) :226-229

[2] Chiral Superstructures of Insulin Amyloid Fibrils [J].

Babenko, Viktoria ;

Harada, Takunori ;

Yagi, Hisashi ;

Goto, Yuji ;

Kuroda, Reiko ;

Dzwolak, Wojciech .

CHIRALITY, 2011, 23 (08) :638-646

[3] Molecular Mechanism of Thioflavin-T Binding to the Surface of β-Rich Peptide Self-Assemblies [J].

Biancalana, Matthew ;

Makabe, Koki ;

Koide, Akiko ;

Koide, Shohei .

JOURNAL OF MOLECULAR BIOLOGY, 2009, 385 (04) :1052-1063

[4] Chiral bias of amyloid fibrils revealed by the twisted conformation of Thioflavin T: An induced circular dichroism/DFT study [J].

Dzwolak, W ;

Pecul, M .

FEBS LETTERS, 2005, 579 (29) :6601-6603

[5] Conformational indeterminism in protein misfolding: Chiral amplification on amyloidogenic pathway of insulin [J].

Dzwolak, Wojciech ;

Loksztejn, Anna ;

Galinska-Rakoczy, Agnieszka ;

Adachi, Rumi ;

Goto, Yuji ;

Rupnicki, Leszek .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (24) :7517-7522

[6] Bifurcated Hydrogen Bonds Stabilize Fibrils of Poly(L-glutamic) Acid [J].

Fulara, Aleksandra ;

Dzwolak, Wojciech .

JOURNAL OF PHYSICAL CHEMISTRY B, 2010, 114 (24) :8278-8283

[7]

Groenning Minna, 2010, J Chem Biol, V3, P1, DOI 10.1007/s12154-009-0027-5

[8] Crystal structure of thioflavin T bound to the peripheral site of Torpedo californica acetylcholinesterase reveals how thioflavin T acts as a sensitive fluorescent reporter of ligand binding to the acylation site [J].

Harel, Michal ;

Sonoda, Leilani K. ;

Silman, Israel ;

Sussman, Joel L. ;

Rosenberry, Terrone L. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (25) :7856-7861

[9] Is Congo red an amyloid-specific dye? [J].

Khurana, R ;

Uversky, VN ;

Nielsen, L ;

Fink, AL .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (25) :22715-22721

[10] The binding of thioflavin-T to amyloid fibrils: localisation and implications [J].

Krebs, MRH ;

Bromley, EHC ;

Donald, AM .

JOURNAL OF STRUCTURAL BIOLOGY, 2005, 149 (01) :30-37