Control genes in quantitative molecular biological techniques:: the variability of invariance

被引：214

作者：

Stürzenbaum, SR ^{[1
]}

Kille, P ^{[1
]}

机构：

[1] Cardiff Univ, Sch Biosci, BIOSI 2, Cardiff, S Glam, Wales

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2001年 / 130卷 / 03期

关键词：

invariant control; housekeeping; GAPDH; cyclophilin; actin; rRNA;

D O I：

10.1016/S1096-4959(01)00440-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The measurement of transcript levels constitutes the foundation of today's molecular genetics. Independent of the techniques used, quantifications are generally normalised using invariant control genes to account for sample handling, loading and experimental variation. All of the widely used control genes are evaluated, dissecting different methodological approaches and issues regarding the experimental context (e.g. development and tissue type). Furthermore, the major sources of error are highlighted when applying these techniques. Finally, different approaches undertaken to assess the invariance of control genes are critically analysed to generate a procedure that will help to discern the best control for novel experiments. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：281 / 289

页数：9

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