A strategy for specific targeting of therapeutic agents to tumour cells of virus-associated cancers

被引:15
作者
Evans, TJ
Brooks, L
Farrell, PJ
机构
[1] ST MARYS HOSP,IMPERIAL COLL SCH MED,LUDWIG INST CANC RES,LONDON W2 1PG,ENGLAND
[2] ST MARYS HOSP,IMPERIAL COLL SCH MED,DEPT MED MICROBIOL,LONDON W2 1PG,ENGLAND
关键词
Epstein-Barr virus; EBNA; Burkitt's lymphoma; nasopharyngeal carcinoma;
D O I
10.1038/sj.gt.3300392
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Expression of viral genes in tumour cells of the virus-associated cancers could provide highly selective ways of targeting expression of therapeutic vectors to the tumour cells. The ubiquitous presence of EBNA-1 in Epstein-Barr virus associated cancers could be used to activate expression constructs containing oriP in the tumour cells. This is demonstrated for a variety of model system including epithelial, cells which would be the target cell type for the treatment of undifferentiated nasopharyngeal carcinoma, a cancer that always contains Epstein-Bar virus in the tumour cells. Combining an oriP/EBNA-1-dependent Epstein-Barr virus Cp promoter with delayed assay of reporter gene, a 108-fold differential was obtained between the activity of a transfected plasmid in cells containing or lacking EBNA-1 expression. This might provide sufficient specificity for a successful in vivo therapeutic strategy.
引用
收藏
页码:264 / 267
页数:4
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