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Synthesis and gene transfection efficacies of PEI-cholesterol-based lipopolymers

被引：108
作者：
Bajaj, Avinash [1 ]
Kondaiah, Paturu [2 ]
Bhattacharya, Santanu [1 ,3 ]
机构：
[1] Indian Inst Sci, Dept Organ Chem, Bangalore 560012, Karnataka, India
[2] Indian Inst Sci, Dept Mol Reprod Dev & Genet, Bangalore 560012, Karnataka, India
[3] JNCASR, Chem Biol Unit, Bangalore 560064, Karnataka, India
来源：
BIOCONJUGATE CHEMISTRY | 2008年 / 19卷 / 08期
关键词：
D O I：
10.1021/bc700381v
中图分类号：
Q5 [生物化学];
学科分类号：
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要：
Nine lipopolymers based on low molecular weight polyethyleneimines (PEI) and cholesterol via an ether linkage between the polymer amine and the cholesterol backbone have been synthesized. Different percentage of cholesterol moieties have been grafted on three types of PEI of molecular weights 800, 1200, and 2000. These lipopolymers were studied for gene transfection activities in HeLa cells. All the lipopolymers were first optimized for enhanced transfection efficacies as coliposomes with DOPE. All lipopolymers are better transfecting agents and highly serum compatible than commercially available PEI-25KDa. Transfection efficacies and serum compatibility of lipopolymers were found to be dependent upon the MW of PEI used for lipopolymer synthesis and percentage of cholesterol grafting on lipopolymers. Cell viability assay showed that PEI-25KDa is highly toxic as compared to all the lipopolymers. Lipopolyplexes were characterized by transmission electron microscopy, which showed the presence of spherical aggregates.
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页码：1640 / 1651
页数：12
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