An interference free amperometric biosensor for the detection of biogenic amines in food products

被引：112

作者：

Carelli, Donatella ^{[1
,2
]}

Centonze, Diego ^{[1
,2
]}

Palermo, Carmen ^{[1
,2
]}

Quinto, Maurizio ^{[1
,2
]}

Rotunno, Taddeo ^{[1
,2
]}

机构：

[1] Univ Foggia, Dipartimento Sci Agroambientali Chim & Difesa Veg, Foggia, Italy

[2] Univ Foggia, BIOAGROMED, Foggia, Italy

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 23卷 / 05期

关键词：

amperometric biosensors; diamino oxidase; biogenic amines; permselective films; platinum electrode; flow injection analysis;

D O I：

10.1016/j.bios.2007.07.008

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this work is reported the development and application of an amperometric biosensor for the determination of total biogenic amines content by using the commercial diamino oxidase (DAO from Porcine kidney E.C. 1.4.3.6) as the biocomponent, entrapped by glutaraldehyde onto an electrosynthesized bilayer film. In order to minimize both the fouling and the interference caused by the direct electrochemical oxidation of both the analytes (i.e., biogenic an-tines) and the common interferents usually present in food products the performances of Pt and Au electrodes and of several electroproduced anti-interferents mono- and bi-layer films were tested. In spite of a very low activity of the commercial DAO, the biosensor displayed a high response, sensitivity in flow experiments, short response time, a good linear response and low detection limits. The excellent anti-interference characteristics allowed the use of the biosensor in screening analysis of food products. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：640 / 647

页数：8

共 35 条

[1] Oxidase enzyme immobilisation through electropolymerised films to assemble biosensors for batch and flow injection analysis [J].