Electrospun biocomposite nanofibers for urea biosensing

被引：123

作者：

Sawicka, K ^{[1
]}

Gouma, P

Simon, S

机构：

[1] SUNY Stony Brook, Dept Chem, Stony Brook, NY 11790 USA

[2] SUNY Stony Brook, Dept Mat Sci & Engn, Stony Brook, NY 11790 USA

[3] SUNY Stony Brook, Dept Pathol, Stony Brook, NY 11790 USA

[4] SUNY Stony Brook, Dept Biochem, Stony Brook, NY 11790 USA

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2005年 / 108卷 / 1-2期

关键词：

electrospinning; biosensing; urea; urease; nanofibers; nanocomposite;

D O I：

10.1016/j.snb.2004.12.013

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Urease E.C.3.5.1.5 acts as a catalyst in the hydrolysis of urea to ammonia and carbon dioxide. Presence of this enzyme can increase the rate of reaction by 10(14). Urea levels are vital in medical diagnosis, environmental and bioindustrial analysis. This paper presents an innovative technique for enzyme immobilization. Nanocomposite fibers of urease and polyvinylpyrrolidone (PVP) were prepared by the electrospinning technique. The nonwoven mat formed has potential as a urea biosensor. Its advancements over prior technology are faster response time, sensitivity to lower concentrations of urea, and a more versatile design. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：585 / 588

页数：4

共 16 条

[1] ENZYME IMMOBILIZATION ON AN EPOXY MATRIX - DETERMINATION OF L-ARGININE BY FLOW-INJECTION TECHNIQUES [J].

ALONSO, A ;

ALMENDRAL, MJ ;

BAEZ, MD ;

PORRAS, MJ ;

ALONSO, C .

ANALYTICA CHIMICA ACTA, 1995, 308 (1-3) :164-169

[2] Conductometric uric acid and urea biosensor prepared from electroconductive polyaniline-poly(n-butyl methacrylate) composites [J].

Castillo-Ortega, MM ;

Rodriguez, DE ;

Encinas, JC ;

Plascencia, M ;

Méndez-Velarde, FA ;

Olayo, R .

SENSORS AND ACTUATORS B-CHEMICAL, 2002, 85 (1-2) :19-25

[3] MULTIANALYTE MINIATURE CONDUCTANCE BIOSENSOR [J].

CULLEN, DC ;

SETHI, RS ;

LOWE, CR .

ANALYTICA CHIMICA ACTA, 1990, 231 (01) :33-40

[4] Metal oxide-coated polymer nanofibers [J].

Drew, C ;

Liu, X ;

Ziegler, D ;

Wang, XY ;

Bruno, FF ;

Whitten, J ;

Samuelson, LA ;

Kumar, J .

NANO LETTERS, 2003, 3 (02) :143-147

[5] A review on polymer nanofibers by electrospinning and their applications in nanocomposites [J].

Huang, ZM ;

Zhang, YZ ;

Kotaki, M ;

Ramakrishna, S .

COMPOSITES SCIENCE AND TECHNOLOGY, 2003, 63 (15) :2223-2253

[6] Virus-based fabrication of micro- and nanofibers using electrospinning [J].

Lee, SW ;

Belcher, AM .

NANO LETTERS, 2004, 4 (03) :387-390

[7] Fabrication of titania nanofibers by electrospinning [J].

Li, D ;

Xia, YN .

NANO LETTERS, 2003, 3 (04) :555-560

[8] Direct fabrication of composite and ceramic hollow nanofibers by electrospinning [J].

Li, D ;

Xia, YN .

NANO LETTERS, 2004, 4 (05) :933-938

[9] Nanobelts of semiconducting oxides [J].

Pan, ZW ;

Dai, ZR ;

Wang, ZL .

SCIENCE, 2001, 291 (5510) :1947-1949

[10] The enzymatic determination of mercury and copper using acid urease. The effects of buffers [J].

Preininger, C .

MIKROCHIMICA ACTA, 1999, 130 (03) :209-214

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