PTGER2 activation induces PTGS-2 and growth factor gene expression in endometrial epithelial cells of cattle

被引：13

作者：

Gao, Long ^{[1
,2
]}

Liu, Bo ^{[1
,2
]}

Mao, Wei ^{[1
,2
]}

Gao, Ruifeng ^{[1
,2
]}

Zhang, Shuangyi ^{[1
,2
]}

Duritahala ^{[1
,2
]}

Fu, Changqi ^{[1
,2
]}

Shen, Yuan ^{[1
,2
]}

Zhang, Ying ^{[1
,2
]}

Zhang, Nan ^{[1
,2
]}

Wu, Jindi ^{[1
,2
]}

Deng, Yang ^{[1
,2
]}

Wu, Xing ^{[3
]}

Cao, Jinshan ^{[1
,2
]}

机构：

[1] Inner Mongolia Agr Univ, Lab Vet Pharmacol, Coll Vet Med, 306 Zhaowuda Rd, Saihan Dist 010018, Huhhot, Peoples R China

[2] Minist Agr, Key Lab Clin Diag & Treatment Tech Anim Dis, Beijing, Peoples R China

[3] Inst Food & Drug Control, Hohhot, Inner Mongolia, Peoples R China

来源：

ANIMAL REPRODUCTION SCIENCE | 2017年 / 187卷

基金：

中国国家自然科学基金;

关键词：

Bovine endometrial epithelial cell; PTGER2; activation; PTGS-2; Growth factors; PROSTAGLANDIN E-2; CYCLOOXYGENASE-2; EXPRESSION; SIGNALING PATHWAYS; VEGF EXPRESSION; UP-REGULATION; IN-VIVO; RECEPTOR; ANGIOGENESIS; CANCER; BETA;

D O I：

10.1016/j.anireprosci.2017.10.005

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

090502 [动物营养与饲料科学];

摘要：

The prostaglandin E2 receptor 2 (PTGER2) is present in the endometrium and its gene expression is accompanied with endometrial growth, however, it is unknown whether there is endometrial repair through stimulation of growth factor gene expression that is promoted by PTGER2 activation in cattle. The aim of this study was to investigate whether PTGER2 activation can induce prostaglandin-endoperoxide synthase-2 (PTGS-2) and growth factor gene expression by activating PKA and ERK signaling pathways in endometrial epithelial cells of cattle. Results demonstrated that the PTGER2 agonist, butaprost, induced cAMP/PKA and ERK activation and up regulated PTGS-2, VEGF, CTGF, TGF-131 and IL-8 gene expression. These activations were less after PTGER2 antagonist, AH6809, treatment. Data suggested that PTGS-2 gene expression was induced by PTGER2 activation through the PKA and ERK pathways. Furthermore, PTGER2 activation promoted several growth factor gene expressions in endometrial epithelial cells. One potential implication of this fmding is that PTGER2 activation in the endometrium of cattle could induce endometrial repair by stimulating VEGF, CTGF, TGF-131 and IL-8 gene expression.

引用

页码：54 / 63

页数：10

共 51 条

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Vascular endothelial growth factor induction by prostaglandin E2 in human airway smooth muscle cells is mediated by E prostanoid EP2/EP4 receptors and SP-1 transcription factor binding sites [J].