Cloning and functional characterisation of the mouse P2X7 receptor

被引：139

作者：

Chessell, IP

Simon, J

Hibell, AD

Michel, AD

Barnard, EA

Humphrey, PPA

机构：

[1] Univ Cambridge, Dept Pharmacol, Glaxo Inst Appl Pharmacol, Cambridge CB2 1QJ, England

[2] Royal Free Hosp, Mol Neurobiol Unit, London NW3 2QG, England

来源：

FEBS LETTERS | 1998年 / 439卷 / 1-2期

关键词：

mouse P2X(7) receptor; P2Z receptor; ATP; pore;

D O I：

10.1016/S0014-5793(98)01332-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a 1785-bp complementary DNA (cDNA) encoding the murine P2X(7) receptor subunit from NTW8 mouse microglial cells. The encoded protein has 80% and 85% homology to the human and rat P2X(7) subunits, respectively. Functional properties of the heterologously expressed murine P2X(7) homomeric receptor broadly resembled those of the P2X(7) receptor in the native cell line. However, marked phenotypic differences were observed between the mouse receptor, and the other P2X(7) receptor orthologues isolated with respect to agonist and antagonist potencies, and the kinetics of formation of the large aqueous pore. (C) 1998 Federation of European Biochemical Societies.

引用

页码：26 / 30

页数：5

共 15 条

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