N-acyl derivatives of arginine and tryptophan isolated from environmental DNA expressed in Escherichia coli

被引：39

作者：

Brady, SF ^{[1
]}

Clardy, J ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

来源：

ORGANIC LETTERS | 2005年 / 7卷 / 17期

关键词：

D O I：

10.1021/ol0509585

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

[GRAPHICS] Heterologous expression of microbial DNA extracted directly from environmental samples (environmental DNA, eDNA) in easily cultured hosts can provide access to natural products produced by previously uncultured bacteria. This report describes the characterization of antibacterially active long-chain N-acyl derivatives of tryptophan and arginine that are produced by eDNA clones hosted in Escherichia coli. The sequencing and subcloning of the proposed N-acyl amino acid synthases (NASs) for each family of natural products are also described.

引用

页码：3613 / 3616

页数：4

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