Involvement of ureides in nitrogen fixation inhibition in soybean

被引:91
作者
Serraj, R
Vadez, V
Denison, RF
Sinclair, TR
机构
[1] Univ Florida, Inst Food & Agr Sci, USDA ARS, Argon Physiol Lab, Gainesville, FL 32611 USA
[2] Univ Calif Davis, Dept Agron & Range Sci, Davis, CA 95616 USA
关键词
D O I
10.1104/pp.119.1.289
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The sensitivity of N-2 fixation to drought stress in soybean (Glycine max Merr.) has been shown to be associated with high ureide accumulation in the shoots, which has led to the hypothesis that N-2 fixation during drought is decreased by a feedback mechanism. The ureide feedback hypothesis was tested directly by measuring the effect of 10 mM ureide applied by stem infusion or in the nutrient solution of hydroponically grown plants on acetylene reduction activity (ARA). An almost complete inhibition of ARA was observed within 4 to 7 d after treatment, accompanied by an increase in ureide concentration in the shoot but not in the nodules. The inhibition of ARA resulting from ureide treatments was dependent on the concentration of applied ureide. Urea also inhibited ARA but asparagine resulted in the greatest inhibition of nodule activity. Because ureides did not accumulate in the nodule upon ureide treatment, it was concluded that they were not directly inhibitory to the nodules but that their influence mediated through a derivative compound, with asparagine being a potential candidate. Ureide treatment resulted in a continual decrease in nodule permeability to O-2 simultaneous with the inhibition of nitrogenase activity during a 5-d treatment period, although it was not clear whether the latter phenomenon was a consequence or a cause of the decrease in the nodule permeability to O-2.
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页码:289 / 296
页数:8
相关论文
共 30 条
[1]   The feedback mechanism of nitrate inhibition of nitrogenase activity in soybean may involve asparagine and/or products of its metabolism [J].
Bacanamwo, M ;
Harper, JE .
PHYSIOLOGIA PLANTARUM, 1997, 100 (02) :371-377
[2]  
DELCASTILLO LD, 1995, PLANT PHYSIOL, V107, P1187, DOI 10.1104/pp.107.4.1187
[3]   MEASUREMENT OF LEGUME NODULE RESPIRATION AND O2 PERMEABILITY BY NONINVASIVE SPECTROPHOTOMETRY OF LEGHEMOGLOBIN [J].
DENISON, RF ;
LAYZELL, DB .
PLANT PHYSIOLOGY, 1991, 96 (01) :137-143
[4]   NITRATE EFFECTS ON NODULE OXYGEN PERMEABILITY AND LEGHEMOGLOBIN - NODULE OXIMETRY AND COMPUTER MODELING [J].
DENISON, RF ;
HARTER, BL .
PLANT PHYSIOLOGY, 1995, 107 (04) :1355-1364
[5]   Soybean petiole ureide response to water deficits and decreased transpiration [J].
deSilva, M ;
Purcell, LC ;
King, CA .
CROP SCIENCE, 1996, 36 (03) :611-616
[6]   Stress-induced declines in soybean N-2 fixation are related to nodule sucrose synthase activity [J].
Gordon, AJ ;
Minchin, FR ;
Skot, L ;
James, CL .
PLANT PHYSIOLOGY, 1997, 114 (03) :937-946
[7]   STEM INFUSIONS ENHANCED METHIONINE CONTENT OF SOYBEAN STORAGE PROTEIN [J].
GRABAU, LJ ;
BLEVINS, DG ;
MINOR, HC .
PLANT PHYSIOLOGY, 1986, 82 (04) :1013-1018
[8]  
HARTWIG UA, 1994, PHYSIOL PLANTARUM, V92, P375, DOI 10.1111/j.1399-3054.1994.tb08824.x
[9]  
HUNT S, 1993, ANNU REV PLANT PHYS, V44, P483, DOI 10.1146/annurev.pp.44.060193.002411
[10]   NITRATE TRANSPORT PATHWAY INTO SOYBEAN NODULES TRACED BY TUNGSTATE AND (NO3-)-N-15 [J].
MIZUKOSHI, K ;
NISHIWAKI, T ;
OHTAKE, N ;
MINAGAWA, R ;
IKARASHI, T ;
OHYAMA, T .
SOIL SCIENCE AND PLANT NUTRITION, 1995, 41 (01) :75-88