Bitter gourd suppresses lipopolysaccharide-induced inflammatory responses

被引：67

作者：

Kobori, Masuko ^{[1
]}

Nakayama, Hirosuke ^{[2
]}

Fukushima, Kenji ^{[3
]}

Ohnishi-Kameyama, Mayumi ^{[1
]}

Ono, Hiroshi ^{[1
]}

Fukushima, Tatsunobu ^{[4
]}

Akimoto, Yukari ^{[1
]}

Masumoto, Saeko ^{[1
]}

Yukizaki, Chizuko ^{[5
]}

Hoshi, Yoshikazu ^{[3
]}

Deguchi, Tomoaki ^{[6
]}

Yoshida, Mitsuru ^{[1
]}

机构：

[1] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

[2] Saga Prefectural Agr Res Ctr, Saga, Japan

[3] Tokai Univ, Sch Agr, Kumamoto 8691404, Japan

[4] Mitsubishi Rayon Co Ltd, Kanagawa 2300053, Japan

[5] Miyazaki Prefectural Food Res & Dev Ctr, Miyazaki 8800303, Japan

[6] Ariake Natl Coll Technol, Fukuoka 8368585, Japan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2008年 / 56卷 / 11期

关键词：

bitter gourd; inflammation; RAW; 264.7; cells; NF kappa B; MAPKs; DNA microarray; linolenoyl-lysophosphatidylcholine; linoleoyl-lysophosphatidylcholine;

D O I：

10.1021/jf800052y

中图分类号：

S [农业科学];

学科分类号：

09 [农学];

摘要：

Bitter gourd (Momordica charantia L.) is a popular tropical vegetable in Asian countries. Previously it was shown that bitter gourd placenta extract suppressed lipopolysaccharide (LPS)-induced TNF alpha production in RAW 264.7 macrophage-like cells. Here it is shown that the butanol-soluble fraction of bitter gourd placenta extract strongly suppresses LPS-induced TNF alpha production in RAW 264.7 cells. Gene expression analysis using a fibrous DNA microarray showed that the bitter gourd butanol fraction suppressed expression of various LPS-induced inflammatory genes, such as those for TNF, IL1 alpha IL1 beta, G1p2, and Ccl5. The butanol fraction significantly suppressed NF kappa B DNA binding activity and phosphorylation of p38, JNK, and ERK MAPKs. Components in the active fraction from bitter gourd were identified as 1-alpha-linolenoyl-lysophosphatidylcholine (LPC), 2-alpha-linolenoyl-LPC, 1-lynoleoyl-LPC, and 2-linoleoyl-LPC. Purified 1-alpha-linolenoyl-LPC and 1-linoleoyl-LPC suppressed the LPS-induced TNF alpha production of RAW 264.7 cells at a concentration of 10 mu g/mL.

引用

页码：4004 / 4011

页数：8

共 26 条

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Beneficial effects and mechanism of action of Momordica charantia juice in the treatment of streptozotocin-induced diabetes mellitus in rat [J].