The mouse Mps1p-like kinase regulates centrosome duplication

被引：182

作者：

Fisk, HA ^{[1
]}

Winey, M ^{[1
]}

机构：

[1] Univ Colorado, Boulder, CO 80309 USA

来源：

CELL | 2001年 / 106卷 / 01期

关键词：

D O I：

10.1016/S0092-8674(01)00411-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The yeast Mps1p protein kinase acts in centrosome duplication and the spindle assembly checkpoint. We demonstrate here that a mouse Mps1p ortholog (esk, which we designate mMps1p) regulates centrosome duplication. Endogenous mMps1p and overexpressed GFP-mMps1p localize to centrosomes and kinetochores in mouse cells. Overexpression of GFP-mMps1p causes reduplication of centrosomes during S phase arrest. In contrast, a kinase-deficient mutant blocks centrosome duplication altogether. Control of centrosome duplication by mMps1p requires a known regulator of the process, Cdk2. Inhibition of Cdk2 prevents centrosome reduplication and destabilizes mMps1p, causing its subsequent loss from centrosomes, suggesting that Cdk2 promotes mMps1p's centrosome duplication function by regulating its stability during S phase. Thus, mMps1p, an in vitro Cdk2 substrate, regulates centrosome duplication jointly with Cdk2.

引用

页码：95 / 104

页数：10

共 45 条

[1] Mps1 is a kinetochore-associated kinase essential for the vertebrate mitotic checkpoint [J].