Brain-specific sequence Hfb1 is a part of the large 3′-untranslated region of the human complexin 2 gene:: New in vitro and in silico data

被引：2

作者：

Dergunova, LV

Raevskaya, NM

Vladychenskaya, IP

Poltaraus, AB

Klimov, EA

Udina, IG

Sulimova, GE

Limborska, SA

机构：

[1] Russian Acad Sci, Inst Mol Genet, Moscow 123182, Russia

[2] Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 117984, Russia

[3] Russian Acad Sci, Vavilov Inst Gen Genet, Moscow 117809, Russia

来源：

MOLECULAR BIOLOGY | 2001年 / 35卷 / 05期

基金：

俄罗斯基础研究基金会;

关键词：

brain-specific cDNAs; human genome; gene expression; chromosomal location; RT-PCR; 3 '-untranslated region; complexin; 2;

D O I：

10.1023/A:1012361901392

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A genomic clone hybridizing with brain-specific sequence Hfb1 was isolated from a chromosome 5 cosmid library. Hfb1 proved to correspond to a new gene exon which codes for a large 3'-untranslated region of the mRNA for synaptic protein complexin 2. Together with the 985-nt Hfb1 cDNA (EMBLY15167) isolated previously from a cDNA library of the frontal cerebral cortex, the primary structure was established for genomic clone Ghfb sized more than 4 kb. A GenBank search revealed complete identity of the 5' end of Ghfb and the 3'-untranslated region (878-933) of the human complexin 2 mRNA. Large transcripts with the 5' end corresponding to the complexin 2 mRNA and the 3' end to Ghfb were detected in total mRNA of the human brain by means of RT-PCR. The size of the 3'-untranslated region of the human complexin 2 mRNA was estimated at 4 kb.

引用

页码：660 / 666

页数：7

共 23 条

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