Fluorescence linked immunosorbant assays using microtiter plates

被引：12

作者：

Velappan, N. ^{[1
]}

Clements, J. ^{[1
]}

Kiss, C. ^{[1
]}

Valero-Aracama, R. ^{[1
]}

Pavlik, P. ^{[1
]}

Bradbury, A. R. M. ^{[1
]}

机构：

[1] Los Alamos Natl Lab, B Div, Los Alamos, NM 87545 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2008年 / 336卷 / 02期

关键词：

ELISA; FLISA; screening; phage antibody; fluorescence; fluorescent proteins;

D O I：

10.1016/j.jim.2008.04.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence methods are widely used in the detection of antibodies and other binding events. However, as a general screening and detection tool in microtiter plates, enzyme linked immunosorbant (ELISA) methods predominate. In this paper we explore all parameters for effective use of fluorescence as a plate based detection method, including which microtiter plates can be used, the most effective means of immobilization, and the use of different fluorescent dyes or fluorescent proteins. These studies indicate that fluorescent immunosorbant assays (FLISA) can be used as effectively as enzymatic method in microtiter plate based screening methods, including the screening of phage antibody selections. (C) 2008 Elsevier B.V. All rights reserved.

引用

收藏

页码：135 / 141

页数：7

相关论文

共 22 条

[1] High-throughput screening of single-chain antibodies using multiplexed flow cytometry [J].

Ayriss, Joanne ;

Woods, Travis ;

Bradbury, Andrew ;

Pavlik, Peter .

JOURNAL OF PROTEOME RESEARCH, 2007, 6 (03) :1072-1082

[2] THE PHYSICAL AND FUNCTIONAL-BEHAVIOR OF CAPTURE ANTIBODIES ADSORBED ON POLYSTYRENE [J].

BUTLER, JE ;

NI, L ;

NESSLER, R ;

JOSHI, KS ;

SUTER, M ;

ROSENBERG, B ;

CHANG, J ;

BROWN, WR ;

CANTARERO, LA .

JOURNAL OF IMMUNOLOGICAL METHODS, 1992, 150 (1-2) :77-90

[3] Adsorption-induced antigenic changes and their significance in ELISA and immunological disorders [J].

Butler, JE ;

Navarro, P ;

Sun, J .

IMMUNOLOGICAL INVESTIGATIONS, 1997, 26 (1-2) :39-54

[4]

Butler John E, 2004, Methods Mol Med, V94, P333

[5] Green fluorescent antibodies:: novel in vitro tools [J].

Casey, JL ;

Coley, AM ;

Tilley, LM ;

Foley, M .

PROTEIN ENGINEERING, 2000, 13 (06) :445-452

[6] Real-time monitoring of the interactions of two-stranded de novo designed coiled-coils:: Effect of chain length on the kinetic and thermodynamic constants of binding [J].

De Crescenzo, G ;

Litowski, JR ;

Hodges, RS ;

O'Connor-McCourt, MD .

BIOCHEMISTRY, 2003, 42 (06) :1754-1763

[7] ISOLATION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR HUMAN C-MYC PROTO-ONCOGENE PRODUCT [J].

EVAN, GI ;

LEWIS, GK ;

RAMSAY, G ;

BISHOP, JM .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (12) :3610-3616

[8] CONSTRUCTION OF SOLID MATRIX ANTIBODY ANTIGEN COMPLEXES CONTAINING SIMIAN IMMUNODEFICIENCY VIRUS-P27 USING TAG-SPECIFIC MONOCLONAL-ANTIBODY AND TAG-LINKED ANTIGEN [J].

HANKE, T ;

SZAWLOWSKI, P ;

RANDALL, RE .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :653-660

[9] Anti-c-myc antibody 9E10:: epitope key positions and variability characterized using peptide spot synthesis on cellulose [J].

Hilpert, K ;

Hansen, G ;

Wessner, H ;

Küttner, G ;

Welfle, K ;

Seifert, M ;

Höhne, W .

PROTEIN ENGINEERING, 2001, 14 (10) :803-806

[10] Structural dynamics of green fluorescent protein alone and fused with a single chain Fv protein [J].

Hink, MA ;

Griep, RA ;

Borst, JW ;

van Hoek, A ;

Eppink, MHM ;

Schots, A ;

Visser, AJWG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (23) :17556-17560