Influence of carbon and nitrogen source on synthesis of NADP+-isocitrate dehydrogenase, methylmalonyl-coenzyme A mutase, and methylmalonyl-coenzyme A decarboxylase in Saccharopolyspora erythraea CA340

Methyimalonyl-coenzyme A (CoA) mutase, methylmalonyl-CoA decarboxylase and NADP(+)-isocitrate dehydrogenase activity profiles were determined in Saccharopolyspora erythraea CA340, an erythromycin producer, grown in glucose or ammonium chloride. Results show that methylmalonyl-CoA mutase and isocitrate dehydrogenase activities were associated with growth, while methylmalonyl-CoA decarboxylase maximal activity was observed at stationary phase. Isocitrate dehydrogenase synthesis was stimulated by glucose and repressed by ammonium; glucose affected negatively the synthesis of methylmalonyl-CoA mutase, whereas methylmalonyl-CoA decarboxylase production was not affected by carbon or nitrogen sources. These results suggested that the negative effect of glucose or ammonium oil erythromycin production could be due, in part to lower pools of succinyl-CoA and methylmalonyl-CoA (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.