A novel phytyltransferase from Synechocystis sp PCC 6803 involved in tocopherol biosynthesis

被引：94

作者：

Schledz, M

Seidler, A

Beyer, P

Neuhaus, G

机构：

[1] Greenovat Pflanzenbiotechnol GmbH, D-79111 Freiburg, Germany

[2] Univ Freiburg, Ctr Appl Biosci, D-79104 Freiburg, Germany

[3] Ruhr Univ Bochum, Fac Biol Biochem Pflanzen, D-44780 Bochum, Germany

来源：

FEBS LETTERS | 2001年 / 499卷 / 1-2期

关键词：

prenyl/phytyltransferase; plastoquinone; vitamin E; tocopherol; Synechocystis;

D O I：

10.1016/S0014-5793(01)02508-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The deduced polypeptide sequence of open reading frame slr1736 reveals homology to chlorophyll synthase and 1,4-dihdroxy-2-naphthoic acid phytyltransferase in Synechocystis sp, strain PCC 6803. In tocopherol and plastoquinone biosynthesis, a condensation reaction mechanistically similar to that of these two enzymes is performed. To analyze the function of this novel prenyltransferase, a deletion mutant of slr1736 was generated bg homologous recombination. The mutant showed a markedly decreased tocopherol content, while plasto-quinone levels remained unchanged. Since the aromatic precursor homogentisic acid accumulated in the mutant, the function of the enzyme was proven to he a novel tocophcrol phytyltransferase. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：15 / 20

页数：6

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