Structure and dynamics of polyunsaturated hydrocarbon chains in lipid bilayers - significance for GPCR function

被引：60

作者：

Gawrisch, Klaus ^{[1
]}

Soubias, Olivier ^{[1
]}

机构：

[1] NIAAA, Sect NMR, Lab Membrane Biochem & Biophys, NIH, Bethesda, MD 20892 USA

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 2008年 / 153卷 / 01期

关键词：

docosahexaenoic acid; docosapentaenoic acid; phosphatidylcholine; phosphatidylethanolamine; phosphatidylserine; G-protein coupled membrane receptor; rhodopsin; nuclear magnetic resonance;

D O I：

10.1016/j.chemphyslip.2008.02.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This review summarizes results of our recent solid-state NMR investigations on polyunsaturated 18:022:6n3-PC/PE/PS and 18:0-22:5n6-PC bilayers. Data on structure and dynamics of the polyunsaturated docosahexaenoyl (DHAn3,22:6n3) and docosapentaenoyl chains (DPAn6,22:5n6), investigated at physiological conditions,are reported. Lipid-protein interaction was studied on reconstituted bilayers containing the G-protein coupled membrane receptor (GPCR) rhodopsin as well as on rod outer segment (ROS) disk membranes prepared from bovine retinas. Results reveal surprisingly rapid conformational transitions of polyunsaturated chains and existence of weakly specific interactions of DHAn3 with spatially distinct sites on rhodopsin. Published by Elsevier Ireland Ltd.

引用

页码：64 / 75

页数：12

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