Orthotopic implantation of colon carcinoma cells provides an experimental model in the rat that replicates the regional spreading pattern of human colorectal cancer

被引：12

作者：

García-Olmo, D ^{[1
]}

García-Rivas, M ^{[1
]}

García-Olmo, DC ^{[1
]}

Atiénzar, M ^{[1
]}

机构：

[1] Hosp Gen Albacete, Serv Cirugia Gen, Res Unit, Albacete 02006, Spain

来源：

CANCER LETTERS | 1998年 / 132卷 / 1-2期

关键词：

experimental colon adenocarcinoma; syngenic rats; orthotopic implantation;

D O I：

10.1016/S0304-3835(98)00174-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Our goal was to develop an experimental model of colon adenocarcinoma based on the orthotopic implantation of tumour cells in syngenic rats which would replicate the pattern of regional spreading of human colorectal adenocarcinoma. We used cell line DHD/K12-PROb and 62 BD-IX rats with intra-caecal injection of 1 x 10(6) cells. Macroscopic and histological examinations were made at different times after injection (from 1 to 16 weeks) with particular emphasis on caecal lesions and rumours were classified according to the TNM system (UICC, 1987). Our results suggest that this model provides a step-by-step reproduction of the development of human colorectal adenocarcinoma. It also allows us to predict how long it will take to achieve a certain degree of local spreading, which is essential for the design of cancer-related experiments. Moreover, our model has the advantage that it uses immunocompetent rats, which facilitates its application. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：127 / 133

页数：7

共 13 条

[1] EXPERIMENTAL-MODEL OF LIVER METASTASES - ADHESION AND GROWTH OF CELLS ON CONTACT WITH ENDOTHELIAL OR HEPATOCYTE CELL MONOLAYER-CULTURES [J].

BAIL, JP ;

FOULTIER, MT ;

PATRICE, T .

RESEARCH IN EXPERIMENTAL MEDICINE, 1994, 194 (01) :53-61

[2]

BAIL JP, 1991, J CHIR-PARIS, V128, P356

[3]

BRESALIER RS, 1987, CANCER RES, V47, P1398

[4]

Dukes C, 1937, Proc R Soc Med, V30, P371

[5] CHARACTERIZATION OF AN ANIMAL-MODEL OF METASTATIC COLON-CARCINOMA [J].

DUNNINGTON, DJ ;

BUSCARINO, C ;

GENNARO, D ;

GREIG, R ;

POSTE, G .

INTERNATIONAL JOURNAL OF CANCER, 1987, 39 (02) :248-254

[6] MODELS OF HUMAN METASTATIC COLON CANCER IN NUDE-MICE ORTHOTOPICALLY CONSTRUCTED BY USING HISTOLOGICALLY INTACT PATIENT SPECIMENS [J].

FU, XY ;

BESTERMAN, JM ;

MONOSOV, A ;

HOFFMAN, RM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (20) :9345-9349

[7]

GOLDROSEN MH, 1980, CANCER, V45, P1223, DOI 10.1002/1097-0142(19800315)45:5+<1223::AID-CNCR2820451330>3.0.CO

[8]

2-F

[9] SELECTION BY TRYPSIN OF 2-SUBLINES OF RAT COLON CANCER-CELLS FORMING PROGRESSIVE OR REGRESSIVE TUMORS [J].

MARTIN, F ;

CAIGNARD, A ;

JEANNIN, JF ;

LECLERC, A ;

MARTIN, M .

INTERNATIONAL JOURNAL OF CANCER, 1983, 32 (05) :623-627

[10]

MORIKAWA K, 1988, CANCER RES, V48, P6863

← 1 2 →