Reversible modulation of quantum dot photoluminescence using a protein-bound photochromic fluorescence resonance energy transfer acceptor

被引:215
作者
Medintz, IL
Trammell, SA
Mattoussi, H
Mauro, JM
机构
[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA
[2] USN, Res Lab, Div Opt Sci, Washington, DC 20375 USA
[3] Mol Probes Inc, Eugene, OR 97402 USA
关键词
D O I
10.1021/ja037970h
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Multiple copies (∼20) of Escherichia coli maltose binding protein (MBP) were coordinated to luminescent semiconductor quantum dots (QDs) via a C-terminal oligohistidine segment. The MBP was labeled with a sulfo-N-hydroxysuccinimide-activated photochromic BIPS molecule (1′,3-dihydro-1′-(2-carboxyethyl)-3,3-dimethyl-6-nitrospiro[2H-1-benzopyran-2,2′-(2H)-indoline]) at two different dye-to-MBP ratios; D/P = 1 and 5. The ability of MBP-BIPS to modulate QD photoluminescence was tested by switching BIPS from the colorless spiropyran (SP) to the colored merocyanine (MC) using white light (>500 nm) or UV light (∼365 nm), respectively. QDs surrounded by MBP-BIPS with D/P = 1 were quenched on average ∼25% with consecutive repeated switches, while QDs surrounded by MBP-BIPS with D/P = 5 were quenched ∼60%. This result suggests a possible use of BIPS-labeled proteins in QD-based nanostructures as part of a threshold switch or other biosensing device. Copyright © 2004 American Chemical Society.
引用
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页码:30 / 31
页数:2
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