Susceptibility of human and non-human cell lines to HCV infection as determined by the centrifugation-facilitated method

被引：8

作者：

Morrica, A

Giorgi, M

Maggi, F

Fornai, C

Vatteroni, ML

Marchi, S

Ricchiuti, A

Antonelli, G

Pistello, M

Bendinelli, M

机构：

[1] Univ Pisa, Virol Sect, Dept Biomed, I-56127 Pisa, Italy

[2] Univ Pisa, Retrovirus Ctr, I-56127 Pisa, Italy

[3] Univ Pisa, Med Clin 1, Gastroenterol Unit, Pisa, Italy

来源：

JOURNAL OF VIROLOGICAL METHODS | 1999年 / 77卷 / 02期

关键词：

HCV; centrifugation-facilitated method; cell cultures;

D O I：

10.1016/S0166-0934(98)00155-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The centrifugation-facilitated inoculation method was used to test 51 human and non-human cell lines for ability to support HCV replication As determined by nested RT-PCR, one fifth of the cell lines tested were virus positive 15 days post inoculation suggesting that the centrifugation-facilitated inoculation is an efficient method for cell infection with HCV. However, Virus production by infected cultures remained of low grade, thus showing that the unknown factors which limit HCV replication in vitro are not overcome by the procedure. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：207 / 215

页数：9

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