An active-site titrant for chymotrypsin, and evidence that azapeptide esters are less susceptible to nucleophilic attack than ordinary esters

被引：20

作者：

Gassman, JM

Magrath, J

机构：

[1] INDIANA UNIV PURDUE UNIV,DEPT CHEM,INDIANAPOLIS,IN 46202

[2] MANKATO STATE UNIV,DEPT CHEM,MANKATO,MN 56002

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1996年 / 6卷 / 15期

关键词：

D O I：

10.1016/0960-894X(96)00308-3

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The p-nitrophenyl ester of N-methoxycarbonylazaphenylalanine reacts with chymotrypsin in 1:1 stoichiometry, and the enzyme concentration can be quantified from the absorbance of the p-nitrophenolate produced in the reaction. This azapeptide does not decompose at pH 7 in the presence or absence of 1 mM hydroxylamine. Copyright (C) 1996 Elsevier Science Ltd

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页码：1771 / 1774

页数：4

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