Repair of injured spinal cord using platelet-rich plasma- and endometrial stem cells-loaded chitosan scaffolds

被引：6

作者：

Ai, Jafar ^{[1
,2
]}

Farzin, Ali ^{[2
]}

Zamiri, Sina ^{[3
]}

Hadjighassem, Mahmoudreza ^{[1
,2
]}

Ebrahimi-Barough, Somayeh ^{[2
]}

Ai, Armin ^{[4
]}

Mohandesnezhad, Sanam ^{[2
]}

Karampour, Ahmad ^{[2
]}

Farahani, Morteza Sagharjoghi ^{[1
]}

Goodarzi, Arash ^{[5
]}

机构：

[1] Univ Tehran Med Sci, Neurosci Inst, Brain & Spinal Cord Injury Res Ctr, Tehran, Iran

[2] Univ Tehran Med Sci, Sch Adv Technol Med, Dept Tissue Engn & Appl Cell Sci, Tehran, Iran

[3] York Univ, Kinesiol & Hlth Dept, Toronto, ON, Canada

[4] Univ Tehran Med Sci, Sch Dent, Tehran, Iran

[5] Fasa Univ Med Sci, Sch Adv Technol Med, Dept Tissue Engn, Fasa, Iran

来源：

INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS | 2021年 / 70卷 / 14期

关键词：

Chitosan scaffold; human endometrial stem cells; platelet-rich plasma; spinal cord injury; FACIAL-NERVE REGENERATION; FUNCTIONAL RECOVERY; BONE ALLOGRAFT; GROWTH-FACTORS; TRANSPLANTATION; COMBINATION; MECHANISMS; DELIVERY;

D O I：

10.1080/00914037.2020.1772257

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

082905 [生物质能源与材料]; 100103 [病原生物学];

摘要：

Engineering scaffolds that can not only act as a vehicle for stem cell delivery but also support axon regeneration, may significantly improve the treatment outcome of spinal cord injury (SCI). In this study, we developed chitosan scaffold containing human endometrial stem cells (hEnSCs) and platelet-rich plasma (PRP) to provide a controlled environment for facilitating cell ingrowth and differentiation and also to persuade axon nervous tissue repair. The results showed that 80 mu L PRP-loaded chitosan scaffold has excellent hEnSCs viability and can promote the regeneration of nerve fibers and angiogenesis and prevent the formation of scar tissue in the repair of SCI.

引用

收藏

页码：1002 / 1011

页数：10

相关论文

共 51 条

[1]

Platelet-rich plasma preparation for regenerative medicine: optimization and quantification of cytokines and growth factors [J].

Amable, Paola Romina ;

Vieira Carias, Rosana Bizon ;

Telles Teixeira, Marcus Vinicius ;

Pacheco, Italo da Cruz ;

Farias Correa do Amaral, Ronaldo Jose ;

Granjeiro, Jose Mauro ;

Borojevic, Radovan .

STEM CELL RESEARCH & THERAPY, 2013, 4

[2]

PCL/gelatin nanofibrous scaffolds with human endometrial stem cells/Schwann cells facilitate axon regeneration in spinal cord injury [J].

Babaloo, Hamideh ;

Ebrahimi-Barough, Somayeh ;

Derakhshan, Mohammad Ali ;

Yazdankhah, Meysam ;

Lotfibakhshaiesh, Nasrin ;

Soleimani, Masoud ;

Joghataei, Mohammad-Taghi ;

Ai, Jafar .

JOURNAL OF CELLULAR PHYSIOLOGY, 2019, 234 (07) :11060-11069

[3]

Angiogenic properties of sustained release platelet-rich plasma: Characterization in-vitro and in the ischemic hind limb of the mouse [J].

Bir, Shyamal Chandra ;

Esaki, Jiro ;

Marui, Akira ;

Yamahara, Kenichi ;

Tsubota, Hideki ;

Ikeda, Tadashi ;

Sakata, Ryuzo .

JOURNAL OF VASCULAR SURGERY, 2009, 50 (04) :870-879

[4]

The Use of Methylprednisolone in Acute Spinal Cord Injury A Review of the Evidence, Controversies, and Recommendations [J].

Breslin, Kristen ;

Agrawal, Dewesh .

PEDIATRIC EMERGENCY CARE, 2012, 28 (11) :1238-1248

[5]

Gradual loss of myelin and formation of an astrocytic scar during Wallerian degeneration in the human spinal cord [J].

Buss, A ;

Brook, GA ;

Kakulas, B ;

Martin, D ;

Franzen, R ;

Schoenen, J ;

Noth, J ;

Schmitt, AB .

BRAIN, 2004, 127 :34-44

[6]

Chitosan-collagen/organomontmorillonite scaffold for bone tissue engineering [J].

Cao, Xianshuo ;

Wang, Jun ;

Liu, Min ;

Chen, Yong ;

Cao, Yang ;

Yu, Xiaolong .

FRONTIERS OF MATERIALS SCIENCE, 2015, 9 (04) :405-412

[7]

Insights into the swelling process and drug release mechanisms from cross-linked pectin/high amylose starch matrices [J].

Carbinatto, Fernanda M. ;

de Castro, Ana Dosis ;

Evangelista, Raul C. ;

Cury, Beatriz S. F. .

ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2014, 9 (01) :27-34

[8]

A randomized trial of pregabalin in patients with neuropathic pain due to spinal cord injury [J].

Cardenas, Diana D. ;

Nieshoff, Edward C. ;

Suda, Kota ;

Goto, Shin-ichi ;

Sanin, Luis ;

Kaneko, Takehiko ;

Sporn, Jonathan ;

Parsons, Bruce ;

Soulsby, Matt ;

Yang, Ruoyong ;

Whalen, Ed ;

Scavone, Joseph M. ;

Suzuki, Makoto M. ;

Knapp, Lloyd E. .

NEUROLOGY, 2013, 80 (06) :533-539

[9]

Cho Hyong-Ho, 2008, [Korean Journal of Otorhinolaryngology Head and Neck Surgery, 대한이비인후과학회지 두경부외과학], V51, P422

[10]

Effect of Neural-Induced Mesenchymal Stem Cells and Platelet-Rich Plasma on Facial Nerve Regeneration in an Acute Nerve Injury Model [J].

Cho, Hyong-Ho ;

Jang, Sujeong ;

Lee, Sang-Chul ;

Jeong, Han-Seong ;

Park, Jong-Seong ;

Han, Jae-Young ;

Lee, Kyung-Hwa ;

Cho, Yong-Bum .

LARYNGOSCOPE, 2010, 120 (05) :907-913

← 1 2 3 4 5 6 →