Aptamer biosensor for protein detection based on guanine-quenching

被引：50

作者：

Wang, Wenjuan

Chen, Chunlai

Qian, Min Xie

Zhao, Xin Sheng ^{[1
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2008年 / 129卷 / 01期

基金：

中国国家自然科学基金;

关键词：

aptamer; protein detection; guanine quenching; thrombin; biosensor; aptamer beacon;

D O I：

10.1016/j.snb.2007.07.125

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Based on fluorescence quenching between guanine bases and fluorophore, a cost-effective biosensor for protein detection is developed. In this biosensor, the label-free protein is detected through variation in fluorescence induced by aptamer conformational change during the recognition between aptamer and protein. Using thrombin-binding aptamer and thrombin as a model system, two design strategies, which are named as hairpin design and duplex design, are developed. Moreover, we investigated how the detection limit is influenced by the modifications, the stem length, and the number of quenching guanine bases in the hairpin design, or the length of the complementary sequence in the duplex design. Our results showed that, to optimize the detection limit, the balance between binding ability of aptamer to protein and quenching efficiency of guanine quenching aptamer have to be achieved. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：211 / 217

页数：7

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