Profiling ethylene-regulated gene expression in Arabidopsis thaliana by microarray analysis

被引:126
作者
Van Zhong, G [1 ]
Burns, JK [1 ]
机构
[1] Univ Florida, IFAS, Ctr Citrus Res & Educ, Dept Hort Sci, Lake Alfred, FL 33850 USA
关键词
Arabidopsis; ethylene regulation; gene expression; microarray;
D O I
10.1023/B:PLAN.0000009270.81977.ef
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ethylene-regulated gene expression in leaves of Arabidopsis thaliana was investigated with an expressed sequence tag-based microarray containing about 6000 unique genes. Comparing expression profiles of the ethylene-insensitive mutant etr1-1, the ethylene-constitutive mutant ctr1-1, ethylene-treated wild-type and untreated wild-type plants identified ca. 7% of the investigated genes as ethylene-regulated. Exogenous ethylene treatment and ctr1-1 had similar changes in gene expression, but differences were noted. Ethylene-regulated genes involved in its own biosynthesis and signal transduction pathway were identified. A large number of transcription factors and some putative signaling components were highly regulated by ethylene. Chloroplast structural protein and photosynthetic genes were generally down-regulated. Ethylene appeared to regulate other primarymetabolic genes. Plant defense and PR protein genes were differentially regulated, with some genes within this class highly up-regulated. Other ethylene-regulated genes identified were known sugar-,auxin-,wounding-and jasmonic acid-related genes, suggesting the existence of coordinated interactions between ethylene and other hormonal and defense signaling pathways. Although hundreds of potentially important transcriptome changes were identified, the functions of many ethylene-regulated genes remain unknown.
引用
收藏
页码:117 / 131
页数:15
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