Serum lysophosphatidic acid concentrations measured by dot immunogold filtration assay in patients with acute myocardial infarction

被引：85

作者：

Chen, X

Yang, XY

Wang, ND

Ding, C

Yang, YJ

You, ZJ

Su, Q

Chen, JH ^{[1
]}

机构：

[1] Peking Univ, Coll Chem, Dept Biol Chem, Minist Educ Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China

[2] Chinese Acad Med Sci, Div Biochem, Cardiovasc Inst, Beijing, Peoples R China

[3] Chinese Acad Med Sci, Fu Wai Hosp, Beijing, Peoples R China

[4] Peking Union Med Coll, Beijing, Peoples R China

来源：

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION | 2003年 / 63卷 / 7-8期

基金：

北京市自然科学基金; 中国国家自然科学基金;

关键词：

acute myocardial infarction (AMI); antibody to LPA; dot immunogold filtration assay (DIFA); lysophosphatidic acid (LPA);

D O I：

10.1080/00365510310003265

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 [基础医学];

摘要：

In this study the relation between lysophosphatidic acid (LPA) and myocardial infarction was investigated, the typical and simplified methods for measuring serum LPA concentration by dot immunogold filtration assay (DIFA) based on a polyclonal antibody to LPA were developed, and serum LPA concentrations were measured in 31 patients with acute myocardial infarction (AMI) and 12 controls (blood donors) by DIFA. Serum LPA levels were raised more than twofold 8 h after the onset of AMI. Maximal elevation (10.43 mg/L) was found at 48-72 h following onset and remained higher than the control concentration (1.66 mg/L) 7 days after AMI. The rise in serum LPA concentration in AMI patients suggests that LPA might be involved in AMI-related pathophysiology in the cardiovascular system. The simplified DIFA developed in the present study for measuring serum LPA concentration is convenient and highly sensitive.

引用

页码：497 / 503

页数：7

共 18 条

[1]

Production and application of LPA polyclonal antibody [J].