The NIT1 promoter allows indu cible and reversible silencing of centrin in Chlamydomonas reinhardtii

被引：24

作者：

Koblenz, B ^{[1
]}

Lechtreck, KF ^{[1
]}

机构：

[1] Univ Cologne, Inst Bot, D-50931 Cologne, Germany

来源：

EUKARYOTIC CELL | 2005年 / 4卷 / 11期

关键词：

D O I：

10.1128/EC.4.11.1959-1962.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An inverted repeat corresponding to parts of the centrin gene of Chlamydomonas reinhardtii was placed downstream of the NIT1 promoter, which is induced by ammonium starvation. After induction, transformants developed centrin deficiency as assayed by immunofluorescence, Western blotting, and Northern blotting. The effect was reversible, demonstrating that the NIT1 promoter allowed controlled RNA interference in Chlamydomonas reinhardtii.

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页码：1959 / 1962

页数：4

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