Interaction of yeast Rvs167 and Pho85 cyclin-dependent kinase complexes may link the cell cycle to the actin cytoskeleton

被引:73
作者
Lee, J [1 ]
Colwill, K [1 ]
Aneliunas, V [1 ]
Tennyson, C [1 ]
Moore, L [1 ]
Ho, YE [1 ]
Andrews, B [1 ]
机构
[1] Univ Toronto, Dept Mol & Med Genet, Toronto, ON, Canada
关键词
D O I
10.1016/S0960-9822(07)00561-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: PHO85 encodes the catalytic subunit of a cyclin-dependent kinase (Cdk) in budding yeast and functions in phosphate and glycogen metabolism. Pho85 associated with the G1 cyclins Pcl2 and Pcl2 is also required for cell cycle progression in the absence of the Cdc28 cyclins Cln1 and Cln2. Loss of Pcl1, Pcl2 and related Pho85 cyclins results in budding defects, suggesting that Pcl-Pho85 complexes function in cell morphogenesis early in the cell cycle; their precise role is not clear, however. Results: To identify targets for Pcl-Pho85 kinases, we performed yeast two-hybrid interaction screens using Pcl2 and the related cyclin Pcl9. We identified RVS167, a gene involved in endocytosis, organization of the actin cytoskeleton, and cell survival after starvation. Like rvs 167 Delta mutants, pho85 mutants or strains deleted for the Pcl1,2-type Pho85 cyclins showed abnormal cell morphology on starvation, sensitivity to salt, random budding in diploids, and defects in endocytosis and in the actin cytoskeleton. Overexpression of Rvs 167 in wild-type cells caused morphological abnormalities and growth arrest at high temperatures; these phenotypes were exacerbated by deleting PHO85. Rvs 167 has a Src homology 3 (SH3) domain and five potential Pho85 phosphorylation sites; recombinant Rvs167 was phosphorylated by the Pcl2-Pho85 kinase in vitro. Maximal phosphorylation of Rvs 167 in vivo required Pho85 and the Pcl1,2-type cyclins. Conclusions: Rvs167 interacts with Pho85 cyclins and is implicated as a target of Pho85 kinases in vive. Our results identify a connection between Cdks and the actin cytoskeleton; interaction of Rvs 167 and Pcl-Pho85 Cdks might contribute to actin cytoskeleton regulation in response to stresses such as starvation. (C) Current Biology Ltd ISSN 0960-9822.
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页码:1310 / 1321
页数:12
相关论文
共 40 条
  • [1] Swi5 controls a novel wave of cyclin synthesis in late mitosis
    Aerne, BL
    Johnson, AL
    Toyn, JH
    Johnston, LH
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1998, 9 (04) : 945 - 956
  • [2] DEFINING PROTEIN INTERACTIONS WITH YEAST ACTIN IN-VIVO
    AMBERG, DC
    BASART, E
    BOTSTEIN, D
    [J]. NATURE STRUCTURAL BIOLOGY, 1995, 2 (01): : 28 - 35
  • [3] The cyclin family of budding yeast: abundant use of a good idea
    Andrews, B
    Measday, V
    [J]. TRENDS IN GENETICS, 1998, 14 (02) : 66 - 72
  • [4] Bai C, 1997, METHOD ENZYMOL, V283, P141
  • [5] ALTERATION OF A YEAST SH3 PROTEIN LEADS TO CONDITIONAL VIABILITY WITH DEFECTS IN CYTOSKELETAL AND BUDDING PATTERNS
    BAUER, F
    URDACI, M
    AIGLE, M
    CROUZET, M
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (08) : 5070 - 5084
  • [6] Amphiphysin I is associated with coated endocytic intermediates and undergoes stimulation-dependent dephosphorylation in nerve terminals
    Bauerfeind, R
    Takei, K
    De Camilli, P
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (49) : 30984 - 30992
  • [7] Botstein D, 1997, MOL CELLULAR BIOL YE, P1
  • [8] Rvs161p interacts with Fus2p to promote cell fusion in Saccharomyces cerevisiae
    Brizzio, V
    Gammie, AE
    Rose, MD
    [J]. JOURNAL OF CELL BIOLOGY, 1998, 141 (03) : 567 - 584
  • [9] YEAST MUTANT AFFECTED FOR VIABILITY UPON NUTRIENT STARVATION - CHARACTERIZATION AND CLONING OF THE RVS161 GENE
    CROUZET, M
    URDACI, M
    DULAU, L
    AIGLE, M
    [J]. YEAST, 1991, 7 (07) : 727 - 743
  • [10] AUTOIMMUNITY IN STIFF-MAN SYNDROME WITH BREAST-CANCER IS TARGETED TO THE C-TERMINAL REGION OF HUMAN AMPHIPHYSIN, A PROTEIN SIMILAR TO THE YEAST PROTEINS, RVS167 AND RVS161
    DAVID, C
    SOLIMENA, M
    DECAMILLI, P
    [J]. FEBS LETTERS, 1994, 351 (01) : 73 - 79